Statistical analyses of the number of Acks signals detected in in situ
hybridization experiments using sections corresponding to the middle brain
parts (n = 5, 5, and 4
for 0, 30, and 60 min, respectively) revealed that there was a significant increase in the number of Acks signals at 30 and 60 min after the bee ball formation in both the Class I and the Class II KCs, and at 60 min after the bee ball formation in the brain area between the DLs and the OLs, and in the OLs (Figure 4S).