Sentences with phrase «patch clamping»

Kodandaramaiah, Boyden and Forest set out to automate a 30 - year - old technique known as whole - cell patch clamping, which involves bringing a tiny hollow glass pipette in contact with the cell membrane of a neuron, then opening up a small pore in the membrane to record the electrical activity within the cell.

Next, Deisseroth required someone skilled at patch clamping, a technique that uses an electrode to record ions passing through cells.

Our studies involve the use of a whole - cell patch clamp, an electrophysiological approach to measure the transmembrane current of a single cell, to identify the effects of herbs on the cardiovascular system.

The systems can also enhance other techniques, such as patch clamp measurements applied in electrophysiology.

In addition to control of lasers, detectors, shutters and fine motion devices, the module can be used to synchronize external stimulation and data collection instruments such as electrodes, patch clamp recording devices and perfusion systems.

After some time during which reprogramming should take place, you start to evaluate the outcome and conduct functional assays, such as patch clamp recordings in case you try to obtain neurons to prove that cells really change their identity.

This module can also be used to synchronize external stimulation and data collection instruments, such as electrodes, patch clamp recording devices, and perfusion systems.

By electrophysiological patch clamp recordings, we probe the response of neuronal model cells to photoexcitation by short light pulses, excluding other pathways than a photoelectrical coupling, to be of passive nature only.

He is also learning patch clamp and will try to use this technique for further research.

Not exact matches

The researchers used two - photon calcium imaging and patch - clamp electrophysiology, two sophisticated technologies that allowed the researchers to record the signals from individual brain cells.

In order to characterize this possible imbalance, we performed in vitro brain slice whole patch - clamp recordings from the Q175 heterozygous knock - in HD mouse model expressing GFP under the control of the D2 receptor promoter to identify dSPNs (GFP - negative) and iSPNs (GFP - positive).

Loaded under whole cell patch - clamp configuration, the caged Ca2 + molecule DM - nitrophen was used to increase [Ca2 +] i rapidly and reversibly in isolated Deiters cells of the organ of Corti.

Kodandaramaiah spent about four months learning the manual patch - clamp technique, giving him an appreciation for its difficulty.

Karel Svoboda, a group leader at the Howard Hughes Medical Institute's Janelia Farm campus, says he believes the technology will be widely adopted, as it removes the barriers that have prevented more researchers from using patch - clamp recording.

Every cell expresses ion channels, but the most common cells to study with patch - clamp techniques...

Try to find successes throughout the day, even ones that seem trivial, like running a well - controlled Western blot that works the first time or patch - clamping a neuron.

Y.C. performed the in vitro patch - clamp experiments.

It's bad enough that they restricted the number of megastructures in the previous patch, but this senseless clamp on superweapons mechanics just perpetuates a trend that will undoubtedly ruin the game in the long run.

Taking finds in ethnological museums as a starting point, he distinguishes between two different concepts of repair: the patched vessels, statuettes and writing tablets in the ethnological collections very openly display their joints and clamps, and with them their histories as objects.