In contrast, Jaber et al. (2014), using
pentobarbital anesthetic, reported that ODS injection in rats had no effect on chorda tympani responses.
Not exact matches
Because barbiturate
anesthetics (e.g.,
pentobarbital) are reported to block 5 - HT3 signaling in heterologous expression systems (Barann et al., 2000; Rüsch et al., 2007), we tested the effects of different
anesthetics (
pentobarbital vs urethane) on chorda tympani responses.
In WT animals, responses to all tastants except 5 mm quinine were larger under urethane anesthesia than with
pentobarbital (Fig. 7B, Table 4), but this difference in response magnitude with
anesthetic did not occur in the KO mice (Fig. 7C, Table 4), with the exception of sucrose.
Further, we show that
pentobarbital and possibly other barbiturate
anesthetics inhibit 5 - HT3 - mediated responses in this system, affecting some taste modalities more than others.
This has not been observed in single - fiber studies of taste specificity in mice (Ninomiya et al., 1982, 1984a, b); however, those studies were performed using
pentobarbital as an
anesthetic, so the 5 - HT3 receptors would have been blocked under those conditions.
Some mice were anesthetized with sodium
pentobarbital, a common
anesthetic used for taste nerve recording (Horio et al., 2011) and used in a recent study on the possible role of 5 - HT1 and 5 - HT3 in taste function (Jaber et al., 2014).
In our study,
pentobarbital inhibited 5 - HT evoked calcium signals in geniculate ganglion neurons and, at
anesthetic levels in mice, significantly inhibits 5 - HT3 function in vivo.
For the
anesthetic doses used in our experiments, plasma and tissue concentrations of intraperitoneally injected
pentobarbital in mice are likely to be at or above the reported IC50 values for 5 - HT3 receptors (Nelson and Halberg, 1973).
Briefly, birds were anesthetized with barbiturate
anesthetic, equithesin (intrapectorally: 0.85 g chloral hydrate / 4.2 ml
pentobarbital / 0.42 g MgSO4 / 6.92 ml propylene glycol / 1.78 ml 100 % ethanol to a total volume of 20 ml with water, then filtered) and secured on a rotary table.
It is true that miniscule levels of
pentobarbital, an
anesthetic used to euthanize animals, have been found in some foods.
Your cat will be given a barbiturate
anesthetic (sodium
pentobarbital) that will quickly result in a loss of consciousness and death.
Your veterinarian will give your pet an overdose of an
anesthetic drug called sodium
pentobarbital, which quickly causes unconsciousness and then gently stops the heartbeat.
When
Pentobarbital sodium
anesthetic became available in 1930, it made veterinarians more confident about entering the abdomen of dogs and cats to perform serious surgery.