Among the functions
performed by antibodies are preventing bacterial invasion of the intestines, neutralizing toxins and killing viruses.
Not exact matches
The old, slow test detected the auto -
antibodies using radioactive materials, took several days, could only be
performed by highly - trained lab staff and cost several hundred dollars per patient.
Similar experiments were
performed demonstrating that the purified human
antibodies also protected immunosuppressed hamsters against lethal disease caused
by Sin Nombre virus.
Dr. Polonis» lab has focused for many years on functional
antibodies elicited
by natural infection and
by vaccination, utilizing samples from multiple natural history cohorts and vaccine studies; her lab is currently
performing an extensive analysis of pseudovirus neutralizing
antibodies in the large RV144 follow - on trial, RV306.
Confirmation of CD44 positive population post treatment was
performed by incubating cell suspension with CD44 primary
antibody (Abcam)(1:500 dilution) followed
by FITC / TRITC tagged secondary
antibody (Abcam)(1:1000 dilution).
Research Focus: I am highly trained in producing and purifying monoclonal
antibodies from Hybridoma cells,
performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines
by PCR and FACS.
Specifically, we will characterize the role of Clone 10 in red blood cell invasion and schizogeny
by performing immunolocalization studies on infected red blood cells, and invasion / growth inhibition assays to determine the role of anti-Clone 10
antibodies in mediating resistance.
Immunofluorescent labeling was
performed using anti-Olig2 (1 ∶ 4000, Chemicon) and anti-GFAP (1 ∶ 400, Cell Signaling) followed
by fluorescently labeled, secondary anti-Ig
antibodies (Alexa 488 and 568 conjugates, Invitrogen) at a 1 ∶ 2000 dilution.
and affinity of
antibody ACI - 5400 were characterized
by a panel of methods: (i) measuring the selectivity for a specific phospho - Tau epitope known to be associated with tauopathy, (ii)
performing a combination of peptide and protein binding assays, (iii) staining of brain sections from mouse preclinical tauopathy models and from human subjects representing six different tauopathies, and (iv) evaluating the selective binding to pathological epitopes on extracts from tauopathy brains in non-denaturing sandwich assays.
Testing of 51 specimens from CFS patients and 56 matched and healthy controls from the US was
performed independently in three laboratories for XMRV DNA
by using several PCR tests and for anti-XMRV
antibodies using different serological assays.
An isotyping ELISA was
performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control
Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.
The most common test is the SNAP test,
performed by your veterinarian to look for
antibodies to FIV.
FIV blood tests
performed by veterinarians measure
antibody levels against the virus, and at present there is no reliable way to distinguish between an animal that has high
antibody levels due to vaccination and one which has been infected
by the FIV virus.
Diagnosis in the U.S. is done using a post-mortem direct fluorescence
antibody test
performed by a state - approved laboratory for rabies diagnosis.
Veterinarians can determine rather easily whether a cat has been exposed to FCoV
by performing a blood test that detects coronavirus
antibodies in the cat, but a positive result just means the cat's been exposed and has developed
antibodies.
A study
performed by vaccine manufacturer Vanguard using a COMBINATION vaccine (one which contains several different vaccines) tested the efficiency of the vaccine in stimulating
antibodies in an immune response.
Coronavirus
antibody tests are not standardized, so the test should be
performed by the same laboratory to allow results from one test to be compared to the next test.