First observed in B - cell lymphoma, recurrent mutations of epigenetic modifier genes have recently been identified in PTCL - NOS. 9 In the present study,
we performed targeted sequencing of the main epigenetic modifier genes in a large cohort of Chinese PTCL - NOS patients.
Not exact matches
«These predictive algorithms seem to do a good job when CRISPR is
performed in cells or tissues in a dish, but whole genome
sequencing has not been employed to look for all off -
target effects in living animals,» says co-author Alexander Bassuk, MD, PhD, professor of pediatrics at the University of Iowa.
Hur added, «To investigate whether Cpf1 had off -
target effects, we
performed whole genome
sequencing using genomic DNA isolated from one Foxn1 mutant mouse and its wild - type sibling.
They identified ALK - positive mesotheliomas by immunohistochemistry; confirmed with fluorescence in situ hybridization; and
performed targeted next - generation
sequencing of tumor DNA and RNA to get a full picture of the exact genetic rearrangement underpinning the disease.
To determine the genetic basis for his exceptional response, researchers at the Dana - Farber Cancer Institute, Stanford University, Brigham and Women's Hospital and elsewhere
performed targeted and whole - exome
sequencing on his leukemic cells.
Several methodologies for
performing DNA
target enrichment prior to next generation
sequencing have been developed and utilized in a growing number of experimental studies.
Here we show that CRISPR / Cas9 systems can have off -
target cleavage when DNA
sequences have an extra base or a missing base at various locations compared with the corresponding RNA guide strandâ $ ¦ Our results suggest the need to
perform comprehensive off -
target analysis by considering cleavage due to DNA and sgRNA bulges in addition to base mismatches.
In REPAIR, the deactivated Cas13b enzyme seeks out a
target sequence of RNA, and the ADAR2 element
performs the base conversion without cutting the transcript or relying on any of the cell's native machinery.
To evaluate whether loss of ABL kinases affected TAZ activity, we
performed chromatin immunoprecipitation (ChIP) analysis using primers for TAZ
targets identified by ChIP
sequencing analysis (42).
Targeted sequencing was
performed on available tumor samples of 125 patients.
We
performed targeted next - generation
sequencing for 120 genes associated with myeloid neoplasms on megakaryocytes isolated from aspirated bone marrow.