Phage researchers» online stomping grounds More about
phages from the American Society of Microbiology
Hilbert and her colleagues isolated
phages from 50 chicken samples purchased from Austrian supermarkets, street markets and butchers.
They found that
the phages from antibiotic - treated mice carried significantly higher numbers of bacterial drug - resistance genes than they would have carried by chance.
There was some variation in the abundance of
phages from healthy bladders vs. those isolated from women with symptoms of urinary tract infections.
The investigators also found that
phages from different women were clearly related.
For now, Adaptive Phage Therapeutics plans to use the AI system at its Maryland office to determine the best course of treatment for a patient, choose the right
phage from its collection, and send the phage to the hospital.
That's because gut
phage from mice treated with one drug carried high levels of genes that confer resistance to different drugs, which means that the phage could serve as backup when bacteria must find ways to withstand a variety of antibiotics.
What's more,
phage from ampicillin - treated mice carried more genes that help bacteria fight off ampicillin and related penicillin - like drugs, while
phage from ciprofloxacin - treated mice carried more genes that help them fight off ciprofloxacin and related drugs.
The researchers demonstrated this by isolating
phage from either antibiotic - treated mice or untreated mice, then adding those phage to gut bacteria from untreated mice.
For example, when the researchers decoded the complete sequence of one extrachromosomal circular
phage from a disease - causing Staphylococcus, they identified a number of genes that may help this strain evade a host's immune system and that could readily spread to other Staphylococcus bacteria.
Not exact matches
It's developing a machine - learning algorithm that uses data
from the genomes of both
phages and bacteria.
Patients in danger of dying
from uncontrollable bacterial infections could find new allies: killer viruses known as
phages.
The antibiotic, Epimerox, targets weaknesses in bacteria that have long been exploited by viruses that attack them, known as
phage, and has even been shown to protect animals
from fatal infection by Bacillus anthracis, the bacteria that causes anthrax.
Professor Mikael Skurnik
from the University of Helsinki has studied bacteriophages and
phage therapy for a long time.
The
phages» DNA contained some of the genes
from another bacteria's immune system.
They do their dirty work by infiltrating bacteria, including disease - causing germs, and destroying them
from within: After latching onto bacteria, the
phages bore inside and hijack the bacteria's genetic machinery, turning them into
phage factories that eventually make so many copies that the cells burst, killing off the host.
A tiny
phage attacks an E. coli cell in this false - color image
from a transmission electron microscope.
Gut viruses called bacteriophage, or
phage (red), harbor resistance genes that shield bacteria
from antibiotics.
But Collins and Sheetal Modi, Ph.D., the lead author of the study and a postdoctoral fellow in Collins» laboratory and at the Wyss Institute, knew that
phage were also abundant in the gut, and that they were adept at ferrying genes
from one bacterium to another.
We assume that
phages acquire resistance genes
from already resistant bacteria and then transfer those genes to other bacteria,» says Hilbert.
The
Phage, a 100 - plus - member «theme camp,» hosts nightly talks on topics ranging
from stem cells to 3 - D modeling.
A new technique of direct binding of radioactive DNA was used to screen a complementary DNA expression library
from the BJAB cell line in lambda gt11
phage to derive molecular cDNA clones representing a putative B lymphocyte - specific octamer binding protein.
The cDNA insert
from this
phage hybridized to messenger RNA found in B lymphocytes, but not in most other cells.
Peptide
phage gathered
from the MDSCs were expanded, enriched and then sequenced to identify predominant peptides.
Phages, shown here surrounding and attacking a bacterial cell, are part of a newly discovered type of immunity that protects mucus - covered human tissue
from bacterial infection.
And even if there were any, for example due to a bacterium changing its surface structures to prevent the virus
from attaching, the new technology makes it possible to develop a suitable
phage against which a bacterium has not yet developed resistance.
Deep sequencing of the VHH
phage libraries generated
from a set of llamas, which received two different immunization protocols, showed that the new VHH and the previously described anti-HIV VHH J3 [26] were induced by immunization (Table 1).
«To solve this problem, we borrowed a tool
from phages themselves: the enzymes these viruses use to break apart a
phage - infected cell to release their progeny,» says Douglas Deutsch, a graduate student in the lab.
Extrachromosomal DNA can include bacteria - infecting viruses, known as
phages, and strands of self - replicating DNA, known as plasmids, often picked up
from other bacteria.
If a yeast cells finds its way
from the lung to the brain via a
phage or other routes, «that's very bad news,» Bartlett says, «because once it gets into the central nervous system it's in heaven.
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was originally discovered by dairy industry researchers seeking to prevent
phages, the viruses that infect bacteria,
from ruining the cultures used to make cheese and yogurt.
Bacteria incorporate small bits of DNA
from phages into their CRISPR region and use that information to fight off the
phages by chewing up their DNA.
With the use of careful
phage isolation procedures and environmental samples
from various locations, such as alternative media usage and modified plaque - picking methods, we isolated potentially novel
phages.
Obtained as a Fab by selection against gp120 IIIB of an antibody
phage display library prepared
from bone marrow of a asymptomatic HIV - 1 seropositive donor.
Carl said that the day Tom woke up
from his coma after
phage therapy began was the happiest day in his career.
And the
phage therapy has really been a miracle for me, and to, to say that it might mean that millions of people are cured
from this in the future is really a privilege.
Human Antibodies
From V - Gene Libraries Displayed on
Phage», Journal of Molecular Biology, 222: 581 - 597, 1991.
By making transgenic, knock - in mice where the endogenous RNA is tagged with the stem loops of the MS2
phage, and bound to the fluorescent coat protein, we can see all these events of mRNA
from birth to death in living tissues.
Also presented are possible alternative approaches to tackling infections, such as
phages (viruses that kill bacteria) sourcing new antibiotic leads
from oceans, soils and host - associated microbiomes in humans, komodo dragons and leafcutter ants.
So, the
phage does have some antibacterial effects and it comes
from a non-dairy culture.
The Life Foundation is a shadowy organization
from Marvel's comics, best known in the «Lethal Protector» storyline — a storyline we know the film is using for inspiration — for kidnapping Venom and using the symbiote to create five symbiote soldiers of their own: Scream,
Phage, Riot, Lasher, and Agony.