Sentences with phrase «phenol extraction»

To generate a full - length unc - 9:: gfp construct, the 14.7 - kb UNC - 9A + B PCR product (representing the 5» end of unc - 9) and the unc - 9:: gfp plasmid were digested separately with Kpn I, digests electrophoresed through low - melt agarose (SeaPlaque GTG agarose, FMC, Rockland, ME, USA), and appropriate fragments purified by phenol extraction and ethanol precipitation.

The appearance of these compounds in the wine mainly comes from the solid parts of the grape (skin and seeds) during winemaking,» explains the University of Seville researcher Julio Nogales, who also says that «we already knew this, but now we can relate the structural composition of the skin and the seeds with the ease with which we can obtain phenols and understand the differences in phenol extraction according to whether we use the external or internal surface of the skin.»

Not exact matches

After discovering the ease of extraction of phenols from the samples, they were characterised spectrally (FTIR and Raman) to understand their structural composition.

The aim of this project is to improve the extraction of compounds from the skin and the seeds of the grape as it has been shown that phenols have health benefits and add quality to wine.

«Extraction of phenols from grape skin, seeds, optimized.»

These techniques study the vibrations that take place in the molecular links from two complementary points of view, allowing the identification of what the most important families of compounds are in the residue that remains after the extraction of phenols.

Following phenol / chloroform extraction and ethanol precipitation, the cDNA was transcribed into biotin - labeled cRNA using T7 polymerase (Ambion MEGAScript T7).

Proteins were digested with Proteinase K (American Bioanalytical, Natick MA) and DNA was purified by phenol - chloroform extraction and ethanol precipitation.