Total RNA was extracted from the perigonadal (epididymal or parametrial) adipose tissue of individual mice using a commercially available acid -
phenol reagent (TRIzol; Invitrogen Corp., Carlsbad, California, USA).
Total RNA was extracted from frozen adipose tissue (100 mg), FACS - isolated cells (> 105), or cultured cells (60 - mm confluent plate) using a commercially available acid -
phenol reagent (TRIzol; Invitrogen Corp.).
The No. 1 scientific paper: Protein measurement with the folin
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