Sentences with phrase «phosphorylation at»

Thus, phosphorylation at this site likely causes steric and electrostatic disruption of this specific Oct4 homodimer - DNA configuration (the PORE homodimer), but not of the MORE homodimer configuration or Oct4 heterodimer complexes.

RIPK2 inhibitor 1 inhibits phosphorylation at serine 176 and tyrosine 474 and possible inhibition of ubiquitination of RIPK2.

ActD caused AKT phosphorylation at Ser473, indicating full activation of AKT.

The p38 - mediated p53 phosphorylation at Ser15 and subsequent p53 induction has been reported to be responsible for apoptosis induced by nitric oxide, hypoxic conditions, DNA - damaging agents, 1 - nitropyrene, and benzo [a] pyrene [30 - 32].

Chiang and Abraham (43) have recently shown that mTOR phosphorylation at Ser2448 is blocked by rapamycin, and this effect is independent of AKT activation status.

GS is covalently modified by phosphorylation at nine serine residues by several protein kinases.

Akt phosphorylation at pS473 was also measured by ELISA (Thermo Fisher Scientific).

Not exact matches

Because a majority of tumors exhibit phosphorylation changes at some point in their development, his lab looked specifically at protein phosphorylation in vesicles.

The next logical step for me work-wise was at the University of California, Riverside, just outside Los Angeles, where I could investigate the physiological phosphorylation of the proteins I had learned to purify.

Adenosine 3», 5» - monophosphate at a concentration of 5 x 10 - 7 mole per liter causes a 400 percent increase in the rate of phosphorylation of histone catalyzed by a partially purified enzyme preparation from rabbit brain.

«Rissman's prior work demonstrated that CRF and its receptors are integrally involved in changes in another AD hallmark, tau phosphorylation,» said William Mobley, MD, PhD, chair of the Department of Neurosciences and interim co-director of the Alzheimer's Disease Cooperative Study at UC San Diego.

The research team, led by Professor Yoshiaki Ito, Senior Principal Investigator at CSI Singapore, found that a modification called phosphorylation made to RUNX3 promotes cancer progression by allowing cell division.

Tim Clausen's group at the Research Institute of Molecular Pathology (IMP) in Vienna identified an analogous system in gram - positive bacteria, where the role of a degradation tag is fulfilled by a little known post-translational modification: arginine phosphorylation.

Researchers at University of California, San Diego School of Medicine have made the surprisingly simple discovery: The phosphorylation of more than 100 secreted proteins is the work of a single enzyme called Fam20C.

G protein — coupled receptor (GPCR)-- dependent activation of protein kinase A (PKA) led to phosphorylation of RCS at Ser55 and increased its binding to CaM.

«Now, we need to better understand how this works at the molecular level so we can perhaps design therapeutics to prevent phosphorylation of that site.»

The phosphorylation of eIF2alpha, which decreases protein synthesis, was previously found at elevated levels in both humans diagnosed with Alzheimer's and in Alzheimer's Disease (AD) model mice.

Specifically, they looked at eIF2alpha phosphorylation and the regulation of protein synthesis in the mice's hippocampus region — the part of the brain responsible for the retrieval of old memories and the encoding of new ones.

Panda and his colleagues hypothesize that normally, PER1 and PER2 are kept synchronized since they have identical phosphorylation sites — they are turned on and off at the same times, keeping sleep and eating cycles aligned.

A key step in many signaling pathways is «phosphorylation,» the activation or deactivation of a protein by adding a phosphate group at certain sites on the protein.

Increased Smad1 / 5 phosphorylation was observed even at a low dose (6 pg) of mutant ACVR1.

«Pin1 changes protein shape through proline - directed phosphorylation, which is a major control mechanism for disease,» explains co-senior author Kun Ping Lu, MD, PhD, Director of Translational Therapeutics in the Cancer Research Institute at BIDMC and Professor of Medicine at Harvard Medical School who co-discovered the enzyme in 1996.

At a molecular level, loss of insulin signaling in astrocytes impaired tyrosine phosphorylation of Munc18c.

In the past 27 years, we have found that this cycling sugar modification of proteins — called O - linked N - acetylglucosamine, or O - GlcNAc — is nearly as common as protein phosphorylation and often competes with it at the same or proximal sites on proteins to regulate nearly every cellular process in response to nutrients and stress.

«I was shocked at the profound effect phosphorylation had on the Huntington's model mice,» said first author Ian Kratter, MD, PhD, a former graduate student at Gladstone and the University of California, San Francisco.

«Phosphorylation helps control how proteins fold and the systems in cells that clear proteins,» explained Finkbeiner, who is a senior investigator at Gladstone.

Phosphorylation of the nuclear form of varicella - zoster virus immediate - early protein 63 by casein kinase II at serine 186.

The regulatory or phosphorylation domain of p120 catenin controls E-cadherin dynamics at the plasma membrane.

«I was shocked at the profound effect phosphorylation had on the Huntington's model mice,» said first author Ian Kratter, MD, PhD, a former graduate student at Gladstone and the University of California, San Francisco (UCSF).

The enhanced hiPS - HEP cells respond to insulin with phosphorylation of protein kinase B - α (Akt), even at low insulin concentrations, and the genes involved in glycogen metabolism, gluconeogenesis, and insulin signaling are expressed at similar levels as in hphep cells.

Stimulation of BP with the Tie2 ligand Ang1 resulted in detectable Tie2 phosphorylation, albeit again at considerably lower levels as in EC (Fig. 1e — h).

Mutation of Hof1 PEST motif phosphorylation sites leads to retention of Hof1 at the bud neck and a decrease in the rate of myosin contraction.

Ipl1p - dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.

Conformational epitopes at cadherin calcium - binding sites and p120 - catenin phosphorylation regulate cell adhesion.

Phosphorylation of Merkel cell polyomavirus large tumor antigen at serine 816 by ATM kinase induces apoptosis in host cells.

We also show that a general repression of translation, mediated by the phosphorylation of translation initiation factor eIF2 at serine 51 (P - eIF2α), is required to maintain the quiescent state.

Here, we show that enhanced hiPS - HEP cells respond to insulin with phosphorylation of protein kinase B - α (Akt), even at low insulin concentrations (Figure 4, Panels E and F), and that the genes involved in glycogen metabolism, gluconeogenesis, and insulin signaling are expressed at similar levels as in hphep cells (Figure 4, Panels B, C, and D).

We previously demonstrated that J147 up - regulates the BDNF pathway in huAPP / PS1 mice following seven months of treatment, and here we show that even when administered at a stage when pathology is advanced, J147 can significantly increase two factors critical for memory formation: CREB phosphorylation and BDNF expression (Figure 3H and 3C, respectively).

(2017) Phosphorylation promotes activation - induced cytidine deaminase activity at the Myc oncogene.

Recent studies have established that nucleophosmin - ALK mediates oncogenesis, at least in part, through phosphorylation / activation of the serine / threonine kinase AKT (4, 5).

However, the PI3K - AKT - mTOR pathway model seems to be more complex, as recent evidence suggests that rictor - mTOR complex directly phosphorylates AKT on Ser473 and facilitates Thr308 phosphorylation by PDK1 in vitro and mTOR is directly phosphorylated by p70S6K at Ser2448.

Trametinib inhibits RAS - mutant MLL - rearranged acute lymphoblastic leukemia at specific niche sites and reduces ERK phosphorylation in vivo

Our results showed that ActD induced the phosphorylation of AKT at Ser473 within 2 minutes.

To exclude this possibility, we constructed a phosphorylation - deficient STAT6 mutant in which the potential phosphorylated tyrosine at position 641 was mutated to a tryptophan (STAT6Y641W).

HDM2 was transcribed by the p53 transcription factor, and the patterns of expression and phosphorylation of HDM2 were parallel to those of p53 at low doses (5 or 10 nM) of ActD treatment.

In contrast, treatment with ActD (10 nM) distinctly induced the expression and phosphorylation of p53 at 3 h, reaching a maximal response at 6 h, and maintaining a high level of p53 for up to 12 h in the HepG2 cells.

Initial empirical testing for c - ABL was carried out via an in vitro kinase approach with purified recombinant kinases at the MRC Protein Phosphorylation and Ubiquitination Unit in Dundee, Scotland.

Despite evidence for divergence at several loci, certain 1a characteristics were preserved including the length of the alternative reading frame / F protein (ARF / F) gene, and a subtype 1a - specific phosphorylation site in NS5A (S349).

In the Hepa - 1c1c7 cells, treatment with ActD (10 nM) distinctly induced the expression and phosphorylation of p53 at 6 h, and a high level of p53 was maintained for up to 12 h (Fig. 1A).

Meanwhile, computational modeling of the effect of the phosphorylation in the structure of the protein, led by co-author Jeetain Mittal at Lehigh, showed that the change in electric charge is what led to the difference in the proteins» likelihood of aggregating.