Sentences with phrase «phosphorylation in»

STAT6 is part of the STAT family of transcription factors, its phosphorylation in response to cytokines and growth factors activates the transcription of many genes involved in the immune system such as interleukin 4 (IL - 4).

Brief calorie restriction increases Akt2 phosphorylation in insulin - stimulated rat skeletal muscle.

A range of NSAIDs, but not paracetamol or non-acidic NSAIDs which have a favourable gastrointestinal tolerability profile, uncoupled oxidative phosphorylation in vitro at micromolar concentrations and inhibited respiration at higher concentrations.

Branched - chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise.

This high demand for energy in the brain is mainly achieved by ATP production during oxidation of glucose or oxidative phosphorylation in the mitochondria.

The body uses molecular oxygen to produce energy via oxidative phosphorylation in the mitochondria.

Previous studies have shown that the closely related Oct1 is dynamically phosphorylated [38], and site - directed mutagenesis of potential phosphorylation sites in Oct4 indicated a potential role for phosphorylation in regulating homodimer complex formation [18].

Meanwhile, computational modeling of the effect of the phosphorylation in the structure of the protein, led by co-author Jeetain Mittal at Lehigh, showed that the change in electric charge is what led to the difference in the proteins» likelihood of aggregating.

The results reveal the central role of protein phosphorylation in the atypical cell cycle regulation of a divergent eukaryote.

The role of reversible phosphorylation in the hormonal control of phenylalanine hydroxylase in isolated rat proximal kidney tubules

As shown in Figure 2A, treatment of BaF3 Aut (V658I) with CMP6 completely blocked phosphorylation of JAK1 and STAT5, while the same treatment did not affect either JAK1 or STAT5 phosphorylation in the BaF3 Aut (F958V) cells.

First, we will use our unique DNA - and chromatin - templated in vitro transcription systems to investigate the direct role of p53 serine18 phosphorylation in sestrin gene activation through modulation of p53 - coactivator interactions.

In line with these observations, all F958 - and P960 - targeting JAK1 mutations showed reduced inhibition of STAT5 phosphorylation in response to CMP6, while all the other mutations had similar IC50 to that of the parental BaF3 cells growing in the presence of IL - 3 (data not shown).

The resistance of the JAK2 Y931C positive cells to inhibitor was associated with persistent JAK2 - phosphorylation in the presence of JAK inhibitor (Figure 3E).

In addition to the mouse cell line, we also examined whether IL - 23 treatment results in ERK1 / 2 phosphorylation in human ILC3s.

These results support the amyloid cascade of tau phosphorylation in AD regarding phosphorylation of tau dependent on beta - amyloid deposition in neuritic plaques, but not of tau in neurofibrillary tangles and threads.

Depletion of ABL kinases does not affect YAP1 protein abundance, localization, or tyrosine phosphorylation in breast cancer cells.

We showed that inactivation of the ABL kinases in breast cancer cells resulted in decreased expression of genes in the JAK / STAT and cytokine / cytokine receptor pathway signatures, which may be due to decreased STAT5A mRNA expression and reduced STAT5 phosphorylation in ABL1 / ABL2 - depleted breast cancer cells.

Trametinib inhibits RAS - mutant MLL - rearranged acute lymphoblastic leukemia at specific niche sites and reduces ERK phosphorylation in vivo

AKT activation contributes to mTOR phosphorylation in ALCL cells.

Because treatment with rapamycin also led to decreased AKT phosphorylation in ALK + ALCL cells in our in vitro study, it is tempting to speculate that an effector protein downstream of mTOR - raptor may contribute directly or indirectly to AKT activation.

Redundant mechanisms prevent mitotic entry following replication arrest in the absence of Cdc25 hyper - phosphorylation in fission yeast.

The work has led him to explore the role of protein phosphorylation in a diverse array of cellular functions, including transformation, cell communication, cell adhesion, cell - cycle regulation, the control of gene expression, and protein degradation.

Co-culture of Tie2 - silenced pericytes with EC resulted in reduced EphA2, EphB2 and EphB4 phosphorylation in the co-culture lysates (Fig. 2h).

VEGF internalization is not required for VEGFR - 2 phosphorylation in bioengineered surfaces with covalently linked VEGF.

HIV - 1 triggers WAVE2 phosphorylation in primary CD4 T cells and macrophages, mediating Arp2 / 3 - dependent nuclear migration.

A role for casein kinase II phosphorylation in the regulation of IRF - 1 transcriptional activity.

Independently of Smads, BMP signaling also activates the p38 MAPK pathway (32 — 34); however, additional assays in COS - 7 cells showed no increase in p38 MAPK phosphorylation in response to ACVR1 (R206H)(data not shown).

Thus, the team has demonstrated a novel, positive role for PKR activation and eIF2 - alpha phosphorylation in human globin RNA splicing, in contrast to the long - standing negative role of this intracellular stress response in protein synthesis.

Because a majority of tumors exhibit phosphorylation changes at some point in their development, his lab looked specifically at protein phosphorylation in vesicles.

Not exact matches

For example, cancer cells tend to use glycolysis in the cell cytoplasm in their metabolism, an ancient system, whereas healthy cells use oxidation - phosphorylation.

Bats have evolved enhanced oxidative phosphorylation pathways in response to the increased metabolic demands of flight.

Analysis of signaling pathways that were affected pointed to changes in mitochondrial function and oxidative phosphorylation, consistent with a number of studies showing perturbations of these pathways in neurodegenerative disorders.

The next challenge is to identify the proteins in the isolated vesicles, and determine their phosphorylation states.

P also enhanced phosphorylation of DARPP - 32 on threonine 34 in the hypothalamus of mice.

Adenosine 3», 5» - monophosphate at a concentration of 5 x 10 - 7 mole per liter causes a 400 percent increase in the rate of phosphorylation of histone catalyzed by a partially purified enzyme preparation from rabbit brain.

Researchers focused on two pathways that produce energy in muscles, glycolysis (sugar metabolism) and mitochondrial oxidative phosphorylation (OXPHOS) in both young and middle - aged rats that were fed a normal diet or a calorie - restricted diet.

In mice with a genetic deletion of DARPP - 32 or with point mutations in phosphorylation sites of DARPP - 32, the effects of D - amphetamine, LSD, and PCP on two behavioral parameters — sensorimotor gating and repetitive movements — were strongly attenuateIn mice with a genetic deletion of DARPP - 32 or with point mutations in phosphorylation sites of DARPP - 32, the effects of D - amphetamine, LSD, and PCP on two behavioral parameters — sensorimotor gating and repetitive movements — were strongly attenuatein phosphorylation sites of DARPP - 32, the effects of D - amphetamine, LSD, and PCP on two behavioral parameters — sensorimotor gating and repetitive movements — were strongly attenuated.

This results in cells having less mitochondrial DNA, decreased mitochondrial membrane potential and a faulty process in cell metabolism called oxidative phosphorylation.

In 1996, Peter Klein of the University of Pennsylvania discovered that one of lithium's biological activities was inhibiting GSK - 3, an enzyme that modifies other proteins by attaching phosphate molecules, a process called phosphorylation.

One way for that to happen is through changes in gene expression, but changes in phosphorylation are equally effective,» explains Beltrao.

The research team used mass spectrometry to compare phosphorylation of proteins from mouse embryonic stem cells with fully functioning GSK - 3 to cells in which the gene encoding GSK - 3 had been deleted.

Phosphorylation, she says, shifts the balance of the flip so that the protein spends more time in the active form.

Phosphorylation of PERK (P - PERK), indicative of ER stress and activation of the unfolded protein response, is evident in ß - cells.

«Rissman's prior work demonstrated that CRF and its receptors are integrally involved in changes in another AD hallmark, tau phosphorylation,» said William Mobley, MD, PhD, chair of the Department of Neurosciences and interim co-director of the Alzheimer's Disease Cooperative Study at UC San Diego.

A variety of neurotransmitters, hormones, and other regulatory agents affect the phosphorylation of specific proteins in their target tissues.

Every cell in the body uses phosphorylation, the process of adding a chemical tag to control a protein's function and fate, such as when it moves from one part of a cell to another or binds to other proteins.

Tim Clausen's group at the Research Institute of Molecular Pathology (IMP) in Vienna identified an analogous system in gram - positive bacteria, where the role of a degradation tag is fulfilled by a little known post-translational modification: arginine phosphorylation.

In a recent study appearing in Nature Publishing Group's Scientific Reports, the researchers focused on the properties of schreibersite and conducted experiments with the mineral to better understand how — in a chemical reaction with the corrosive effects of water called «phosphorylation» — schreibersite could have provided the phosphate important to the emergence of early biological lifIn a recent study appearing in Nature Publishing Group's Scientific Reports, the researchers focused on the properties of schreibersite and conducted experiments with the mineral to better understand how — in a chemical reaction with the corrosive effects of water called «phosphorylation» — schreibersite could have provided the phosphate important to the emergence of early biological lifin Nature Publishing Group's Scientific Reports, the researchers focused on the properties of schreibersite and conducted experiments with the mineral to better understand how — in a chemical reaction with the corrosive effects of water called «phosphorylation» — schreibersite could have provided the phosphate important to the emergence of early biological lifin a chemical reaction with the corrosive effects of water called «phosphorylation» — schreibersite could have provided the phosphate important to the emergence of early biological life.

Prior work on nucleoside phosphorylation has shown that inorganic phosphate can serve as both a catalyst and a reactant in nucleoside synthesis, they said.