Sentences with phrase «phosphotyrosine containing»
On the next slide I show you basically the key domain that's involved in this signaling interaction, so when the insulin receptor is phosphorylated, in order to recruit adaptors such as IRS - 1, it uses an important domain called the PTP domain, or phosphotyrosine containing peptides that can actually mediate this interaction, and lead to the interaction of these various molecules within the pathway.
http://www.abcam.com/ Not exact matches
METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.