Sentences with word «photoactivation»

Dr. Lakadamyali states that «at the moment we have to work with imperfect fluorescent proteins and take into account the limitations when quantifying protein stoichiometry, however, now that there is a robust way to characterize photoactivation efficiency, future fluorescent protein engineering work will likely focus on optimizing this parameter for generating fluorescent proteins better suited for molecular counting using super-resolution».

Nguyen D, Mohan M, Elsässer SJ, Uttamapinant C, Chin JW, «Genetic encoding of photocaged cysteine allows photoactivation of TEV protease in live mammalian cells ``, JACS (2014)

Instead, the new method, called patterned photoactivation non-linear SIM, begins by switching on just a subset of fluorescent labels in a sample with a pattern of light.

The team used patterned photoactivation non-linear SIM to produce videos showing structural proteins break down and reassemble themselves as cells move and change shape, as well as the dynamics of tiny pits on cell surfaces called caveolae.

Organic synthesis & chemical probe development, NMR spectroscopy, lipid biochemistry, photoaffinity probes, live cell photoactivation experiments, live cell imaging

Indeed, within the world of super resolution microscopy, PA - FPs have helped create several new imaging methods, including photoactivation - localization microscopy (PALM, also FPALM)(11).

A high contrast ratio usually indicates that a PA - FP has a low level of spontaneous fluorescence in the absence of photoactivation resulting in an increased signal to noise ratio.

«Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.»

The best known reversible PA - FP is the naturally occurring Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation)(6).

Another important parameter to consider is the contrast ratio - a comparison of PA - FP brightness before and after photoactivation.

PA - FPs are divided into 2 main categories - those with irreversible and reversible photoactivation - and have enabled sophisticated imaging techniques.

[Praveen R. Arany et al, Photoactivation of Endogenous Latent Transforming Growth Factor — β1 Directs Dental Stem Cell Differentiation for Regeneration] Could a light - based treatment for cavities and chipped teeth eventually be in the offing for people?

Photoactivation experiments indicate that microvillar actin is mobilized at the lamellipodium, allowing optimal migration.

Patterned photoactivation non-linear SIM captures the 25 images that go into a final reconstruction in about one - third of a second.

In a recent study carried out at ICFO, the Institute of Photonic Sciences, the research group of Advanced fluorescence imaging and biophysics, led by Nest Fellow Dr. Melike Lakadamyali was able to quantify the photoactivation efficiency of all the known «ir - reversibly photoswitching fluorescent proteins» and establish a proper detailed reference framework for determining protein stoichiometry.

Therefore, they emphasized the importance of taking into account the photoactivation efficiency to correctly interpret the quantitative information.

By measuring the photoactivation efficiency as well as other photophysical factors such as blinking, the group determined which proteins are best suited for molecular counting and found that none are perfect (most fluorescent proteins fail to photoactivate about 50 % of the time).

FRET, FRAP, mitochondrial and plasma membrane potential measurements, photoactivation / uncaging, etc..

Common uses of Vector include FRAP, photoactivation, photoablation and uncaging.

If the light intensity needed for activation is too high, the photoactivation step can actually harm your cells before your experiment even begins!

For instance, exposure to light causes release of NO from mitochondria and also an increase in NO levels due to photoactivation of nitric oxide synthase.