Sentences with word «photobleaching»

Fluorescence recovery after photobleaching of GFP - tagged wild - type and S39A fascin showed that dephosphorylated fascin underwent rapid cycles of association to and dissociation from actin filaments in filopodia, with t (1/2) < 10 s.

Furthermore 3i proprietary TTL Synchronization electronics and SlideBook software allow blanking of the illuminating laser light in the time between camera exposures resulting in the minimum possible unwanted photobleaching of cells.

A suite of features helps minimize phototoxicity and photobleaching when capturing 3D images, prolonging the viability of live cells during time - lapse imaging.

If you are performing multicolor experiments, you will want to be sure that the light used to activate the first PA - FP does not photobleach the second, and vice versa.

The label might not find the protein efficiently, or might photobleach over time.

Single - molecule fluorescence spectroscopy of a multichromophoric conjugated polymer (molecular weight ∼ 20,000) revealed surprising single - step photobleaching kinetics and acute jumps in fluorescence intensity.

The thinness of the sheet leads to high axial resolution and negligible photobleaching and background outside of the focal plane, while its simultaneous illumination of the entire field of view permits imaging at hundreds of planes per second even at extremely low peak excitation intensities.

Following up on this observation, the authors show that this process is not simple photobleaching, but rather is caused by active elimination of pigment cells.

Particularly, when coupling SM fluorescence detection with optical tweezers, the reduction of noise and photobleaching from the trapping infrared lasers and the control of biochemical buffers for assembly of the biological complexes and performance of the experimental measurements are of paramount importance.

For PALM, a small area containing inactive PA - FPs is photoactivated, imaged, and then photobleached.

Similar to FPs, PA - FPs allow for non-invasive labelling, but in contrast, PA - FPs offer a reduced potential for photobleaching and phototoxicity that standard FPs might induce though their continuous excitation.

The real time controller (U-RTCE) synchronizes the laser and camera with microsecond illumination accuracy to reduce photobleaching and phototoxicity, helping cells remain healthy during complex experiments.

The timing and dynamics of centromere assembly before and after replication will be determined using fluorescently tagged CENP - A variants and photobleaching methods (FRAP and FLIP) or with tetra - cys CENP - A and fluorescent arsenic compounds (FLASH).

Several methods to measure FRET by fluorescence microscopy can be used, including fluorescence lifetime imaging, spectral imaging, donor fluorescence dequenching upon acceptor photobleaching.

His studies have also utilized fluorescence recovery after photobleaching (FRAP) and fluorescence resonance energy transfer (FRET) to understand the dynamics of these assemblies and their organization.

However, they also continually dose the sample from top to bottom with excitation light which can lead to photobleaching when acquiring multiple images over a large axial range.

«The massive advantage with our stain-less laser - based imaging approaches is that you can use the stem cell sample without having to interrupt the developmental process in real time, you don't need to perform any cell disruption and there is no photobleaching (fading) which is fairly common with fluorescent material,» Catarina enthused.

Scientists are therefore using so - called «FRAP» assays (FRAP: Fluorescence Recovery After Photobleaching) to investigate diffusion kinetics, a method established more than 40 years ago.

Photobleaching and phototoxicity are typically reduced by one to two orders of magnitude relative to that seen with a 1D scanned Bessel beam or the point array scanned excitation of spinning disk confocal microscopy.

Photobleaching is a photochemical alteration process that decreases the fluorescence signal intensity.

These include methods for deconvolution in fluorescence microscopy as applied to cellular morphogenesis, in addition to methods for live - cell binding measurements using fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and single molecule tracking (SMT) as applied to transcription factor regulation.

The Fluorescence Recovery After Photobleaching (FRAP) assay has been used to validate many of the SGC's chemical probes against bromodomains.

In FRAP assays (FRAP: Fluorescence Recovery After Photobleaching), the time that fluorescent molecules need to replenish a bleached - out area is measured,...

- Optogenetics - Uncaging - Photobleaching / FRAP - Photoconversion - Laser ablation - Laser T - Jump - Deep - UV microscopes with transmission down to 230nm Products include off - the - shelf and customized systems for localized illumination in microscopy.

However, the excitation light excites all of the fluorophores as it passes through the specimen, which often leads to photobleaching.

BAZ2 - ICR also shows accelerated Fluorescence recovery after photobleaching (FRAP) recovery at 1 μM in the BAZ2A FRAP assay.

2 - photon imaging, which restricts excitation and photobleaching to the focal plane can be used to overcome this (8).

To investigate whether BAZ2 - ICR can displace BAZ2 bromodomains from chromatin in living cells, we performed a fluorescence recovery after photobleaching (FRAP) assay utilizing GFP - tagged BAZ2A full length protein transfected into human osteosarcoma cells (U2OS).

JQ1 inhibits recruitment and binding of Brd4 to TNFα and E-selectin promoter elements, and accelerates recovery time in FRAP (fluorescence recovery after photobleaching) assays using GFP - Brd4.

Both are capable of collecting transmitted light as well as epifluorescence data and can be used for time - lapse imaging, Fluorescence Resonance Energy Transfer (FRET), and Fluorescence Recovery After Photobleaching (FRAP) experiments.

FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting cyan - excitable donors, phototoxicity.