Sentences with phrase «photobleaching methods»
Not exact matches
Scientists are therefore using so - called «FRAP» assays (FRAP: Fluorescence Recovery After Photobleaching) to investigate diffusion kinetics, a method established more than 40 years ago.
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These include methods for deconvolution in fluorescence microscopy as applied to cellular morphogenesis, in addition to methods for live - cell binding measurements using fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and single molecule tracking (SMT) as applied to transcription factor regulation.
Several methods to measure FRET by fluorescence microscopy can be used, including fluorescence lifetime imaging, spectral imaging, donor fluorescence dequenching upon acceptor photobleaching.