Sentences with phrase «photobleaching of»

Fluorescence recovery after photobleaching of GFP - tagged wild - type and S39A fascin showed that dephosphorylated fascin underwent rapid cycles of association to and dissociation from actin filaments in filopodia, with t (1/2) < 10 s.

Furthermore 3i proprietary TTL Synchronization electronics and SlideBook software allow blanking of the illuminating laser light in the time between camera exposures resulting in the minimum possible unwanted photobleaching of cells.

Not exact matches

Estimation of excited - state absorption and photobleaching in Fe2 + - doped lithium sodium silicate glass under exposure to high - power nanosecond laser pulses

Single - molecule fluorescence spectroscopy of a multichromophoric conjugated polymer (molecular weight ∼ 20,000) revealed surprising single - step photobleaching kinetics and acute jumps in fluorescence intensity.

Photobleaching and phototoxicity are typically reduced by one to two orders of magnitude relative to that seen with a 1D scanned Bessel beam or the point array scanned excitation of spinning disk confocal microscopy.

Following up on this observation, the authors show that this process is not simple photobleaching, but rather is caused by active elimination of pigment cells.

The Fluorescence Recovery After Photobleaching (FRAP) assay has been used to validate many of the SGC's chemical probes against bromodomains.

JQ1 inhibits recruitment and binding of Brd4 to TNFα and E-selectin promoter elements, and accelerates recovery time in FRAP (fluorescence recovery after photobleaching) assays using GFP - Brd4.

Both are capable of collecting transmitted light as well as epifluorescence data and can be used for time - lapse imaging, Fluorescence Resonance Energy Transfer (FRET), and Fluorescence Recovery After Photobleaching (FRAP) experiments.

FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting cyan - excitable donors, phototoxicity.