Sentences with phrase «photoreceptor outer»

In RCD3 affected dogs normal rod - mediated ERG responses fail to develop, photoreceptor outer segments do not reach maturity and rod cells are lost by apoptosis [5].

Phagocytosis of photoreceptor outer segments was defined by the inclusion of rhodopsin - labelled material within the TRA -1-85-bordered intracellular compartment of grafted human iPS - RPE cells.

Importantly, akin to HESC - RPE in vivo, cells at the outside edge of the iPS - RPE cell bolus could phagocytose photoreceptor outer segments from the RCS rat, as indicated by the presence of rhodopsin - positive photoreceptor material within the cellular membrane of iPS - RPE labelled with HSM (Fig. 5E).

Photoreceptor outer segments (POS) were isolated from freshly slaughtered porcine eyes using a continuous sucrose gradient as previously described [8], [32].

Phagocytosis of fluorescently labelled photoreceptor outer segments (green) by iPS - RPE cells in vitro.

(A) Confocal images showing phagocytosis of isolated FITC - labelled porcine photoreceptor outer segments (POS — green) by iPS - RPE in culture.

iPS - RPE were able to phagocytose fluorescently labelled porcine photoreceptor outer segments (POS) in co-culture.

The progression of retinal dystrophy in the RCS rat is such that by 13 weeks post-graft most of the ONL has disappeared [33] and the photoreceptor outer segment layer is reduced to a debris zone [40], a finding we observed in dystrophic controls.

(B - i) Electron microscopy image of a porcine photoreceptor outer segment (POS) adjacent to an iPS - RPE cell following 3 hours co-culture with a porcine retina explant.

Qualitative examination of the host anatomical response to the presence of hNPCctx or hNPCctx - GDNF revealed substantial preservation of the photoreceptor outer nuclear layer (ONL) overlying all subretinal donor cells (Figure 5E and F), with photoreceptor rescue gradually declining outside the distribution of the transplanted cells (Figure 5E and G).

In this well - studied model of autosomal recessive retinitis pigmentosa and secondary photoreceptor degeneration, a MERTK mutation in the RPE compromises their ability to phagocytose shed photoreceptor outer segments [35]--[37].

Rapid formation of all - trans retinol after bleaching in frog and mouse rod photoreceptor outer segments.

Rim formation is not a prerequisite for distribution of cone photoreceptor outer segment proteins.

Interphotoreceptor retinoid - binding protein is the physiologically relevant carrier that removes retinol from rod photoreceptor outer segments.

Lack of protein - tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy.

Not exact matches

«This study for the first time shows increased expression of IL - 33 in AMD and further demonstrates a role for glia - derived IL - 33 in the accumulation of myeloid cells in the outer retina, loss of photoreceptors, and functional impairment of the retina in preclinical models of retina stress,» the authors note.

In wild - type, retinal ganglion cell layer (GCL), inner nuclear layer (INL), inner plexiform layer (IPL), and nuclear layers of rod and cone photoreceptors are distinct, and rod outer segment (OS) is observed at the outer-most layer of the retina.

(A) Rosettes (arrowheads) and pseudo outer nuclear layers (arrow) are detected in explants triple stained for nuclei (blue; DAPI), cone (green; Calbindin), and rod photoreceptors (red; XAP2).

When transplanted to the subretinal space of mice lacking functional photoreceptors, human embryonic stem cells directed toward a retinal lineage integrate into the outer nuclear layer, express photoreceptor markers, and restore a light response as determined by the electroretinogram (ERG)[5].

Reduction of all - trans retinal to all - trans retinol in the outer segments of frog and mouse rod photoreceptors.

Rod and cone photoreceptors in the outer retina transduce light into electrical signals.

Oil droplets, outer, and inner segments were observed in cells expressing photoreceptor specific markers (Figure 7B and 7C).

Effect of Rds abundance on cone outer segment morphogenesis, photoreceptor gene expression, and outer limiting membrane integrity.

Microfluorometric Measurement of the Formation of All - Trans - Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors.

(E) Low power view of a retina section obtained from the same eye used in panel A showing extensive rescue of photoreceptors within the outer nuclear layer (ONL) after subretinal injection of hNPCctx — GDNF.

Fresh porcine retinal tissue was dissected, orientated on a 0.45 mm filter with the photoreceptor cell surface uppermost and placed on top of the iPS - RPE monolayer so that outer segments were adjacent to the RPE.

Conservation of visual acuity in the iPS - RPE transplanted eyes was associated with the preservation of photoreceptors in the host outer nuclear layer (ONL — Fig. 7A), identified by the expression of rhodopsin in the outer segments of photoreceptors (Fig. 7A inset, Dystrophic + transplant).

The gene product's precise role is not currently understood but it is thought to anchor regulatory complexes at the photoreceptor connecting cilium, which acts as a bridge between the inner and outer segments of photoreceptor cells [43] as well as having functions in disk morphogenesis [42] and in the structure of the ciliary axoneme [44].

Layers of the retina: RPE, retinal pigment epithelium; PR, photoreceptors; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; NFL, nerve fiber layer.