In RCD3 affected dogs normal rod - mediated ERG responses fail to develop,
photoreceptor outer segments do not reach maturity and rod cells are lost by apoptosis [5].
Phagocytosis of
photoreceptor outer segments was defined by the inclusion of rhodopsin - labelled material within the TRA -1-85-bordered intracellular compartment of grafted human iPS - RPE cells.
Importantly, akin to HESC - RPE in vivo, cells at the outside edge of the iPS - RPE cell bolus could phagocytose
photoreceptor outer segments from the RCS rat, as indicated by the presence of rhodopsin - positive photoreceptor material within the cellular membrane of iPS - RPE labelled with HSM (Fig. 5E).
Photoreceptor outer segments (POS) were isolated from freshly slaughtered porcine eyes using a continuous sucrose gradient as previously described [8], [32].
Phagocytosis of fluorescently labelled
photoreceptor outer segments (green) by iPS - RPE cells in vitro.
(A) Confocal images showing phagocytosis of isolated FITC - labelled porcine
photoreceptor outer segments (POS — green) by iPS - RPE in culture.
iPS - RPE were able to phagocytose fluorescently labelled porcine
photoreceptor outer segments (POS) in co-culture.
In this well - studied model of autosomal recessive retinitis pigmentosa and secondary photoreceptor degeneration, a MERTK mutation in the RPE compromises their ability to phagocytose shed
photoreceptor outer segments [35]--[37].
Rapid formation of all - trans retinol after bleaching in frog and mouse rod
photoreceptor outer segments.
Interphotoreceptor retinoid - binding protein is the physiologically relevant carrier that removes retinol from rod
photoreceptor outer segments.
Lack of protein - tyrosine sulfation disrupts
photoreceptor outer segment morphogenesis, retinal function and retinal anatomy.
Rim formation is not a prerequisite for distribution of cone
photoreceptor outer segment proteins.
(B - i) Electron microscopy image of a porcine
photoreceptor outer segment (POS) adjacent to an iPS - RPE cell following 3 hours co-culture with a porcine retina explant.
The progression of retinal dystrophy in the RCS rat is such that by 13 weeks post-graft most of the ONL has disappeared [33] and
the photoreceptor outer segment layer is reduced to a debris zone [40], a finding we observed in dystrophic controls.
Not exact matches
In wild - type, retinal ganglion cell layer (GCL), inner nuclear layer (INL), inner plexiform layer (IPL), and nuclear layers of rod and cone
photoreceptors are distinct, and rod
outer segment (OS) is observed at the
outer-most layer of the retina.
Reduction of all - trans retinal to all - trans retinol in the
outer segments of frog and mouse rod
photoreceptors.
Oil droplets,
outer, and inner
segments were observed in cells expressing
photoreceptor specific markers (Figure 7B and 7C).
Effect of Rds abundance on cone
outer segment morphogenesis,
photoreceptor gene expression, and
outer limiting membrane integrity.
Microfluorometric Measurement of the Formation of All - Trans - Retinol in the
Outer Segments of Single Isolated Vertebrate
Photoreceptors.
Fresh porcine retinal tissue was dissected, orientated on a 0.45 mm filter with the
photoreceptor cell surface uppermost and placed on top of the iPS - RPE monolayer so that
outer segments were adjacent to the RPE.
Conservation of visual acuity in the iPS - RPE transplanted eyes was associated with the preservation of
photoreceptors in the host
outer nuclear layer (ONL — Fig. 7A), identified by the expression of rhodopsin in the
outer segments of
photoreceptors (Fig. 7A inset, Dystrophic + transplant).
The gene product's precise role is not currently understood but it is thought to anchor regulatory complexes at the
photoreceptor connecting cilium, which acts as a bridge between the inner and
outer segments of
photoreceptor cells [43] as well as having functions in disk morphogenesis [42] and in the structure of the ciliary axoneme [44].