The phrase
"plane illumination" means shining light directly onto an object from one direction in a flat, even manner.
Full definition
She was making use of the newest version of a breakthrough technique that Betzig unveiled in 2011, called Bessel
beam plane illumination microscopy.
LUXENDO is a spin - off from EMBL in Heidelberg, commercializing fast, advanced
Single Plane Illumination Microscopes (SPIM).
The new multi-view approach helps improve a technique the researchers previously developed called dual -
view plane illumination microscopy (diSPIM).
In 2004 Jan Huisken of the Max Planck Institute of Molecular Cell Biology and Genetics and colleagues published a paper establishing
structured plane illumination microscopy, or SPIM, and the field has been booming ever since.
The «brilliantly simple» idea, as Betzig calls it, of using a sheet of light to reduce cell damage is
called plane illumination microscopy.
The technique, called microenvironmental
selective plane illumination microscopy (meSPIM), uses exceptionally long, thin beams of laser light to trigger fluorescence in a sample, causing it to glow.
We used our single
plane illumination microscope (SPIM) to record the first non-invasive long - term fluorescence live imaging study of the fruit fly Drosophila melanogaster in 2004.
The second microscope, described in a paper published in Nature Biotechnology online on October 13, builds on selective
plane illumination microscopy (SPIM).
AFRICAN GREEN MONKEY CELL: An African green monkey kidney cell expressing the gene c - src, imaged using Bessel beam
plane illumination.
3D CELL DIVISION: The stages of cell division, seen frequently in 2 - dimensions, imaged with the Bessel beam
plane illumination: chromosomes appear in orange and microtubules in green.
Marianas LightSheet ™ merges the low phototoxicity and large specimen handling of dual inverted selective
plane illumination (diSPIM) with the power and flexibility of a live - cell microscope system.
Selective
plane illumination (SPIM) uses a thin sheet of light to illuminate only the plane of interest, reducing phototoxicity by drastically cutting total light dose and allowing for prolonged specimen imaging.
After this initial application, Stelzer's group described the single - plane or selective -
plane illumination microscope (SPIM).