The second microscope, described in a paper published in Nature Biotechnology online on October 13, builds on selective
plane illumination microscopy (SPIM).
In 2004 Jan Huisken of the Max Planck Institute of Molecular Cell Biology and Genetics and colleagues published a paper establishing structured
plane illumination microscopy, or SPIM, and the field has been booming ever since.
The «brilliantly simple» idea, as Betzig calls it, of using a sheet of light to reduce cell damage is called
plane illumination microscopy.
She was making use of the newest version of a breakthrough technique that Betzig unveiled in 2011, called Bessel beam
plane illumination microscopy.
Betzig says Bessel beam
plane illumination microscopy is «ready» for commercialization.
The technique, called microenvironmental selective
plane illumination microscopy (meSPIM), uses exceptionally long, thin beams of laser light to trigger fluorescence in a sample, causing it to glow.