Transient assays were done in onion epidermal cells using
the plant signal sequence fused to RBP and a GFP reporter (ssRBP - GFP fusion protein) with RBP fused to GFP as a control.
Not exact matches
But this part of the project became «almost insignificant» when he discovered a
sequence - specific silencing
signal spreading through a
plant, Voinnet says.
Our discovery that this conservation also extends to the level of HK transmembrane
signaling allowed us to design a test system, whereby transmembrane fusions can be rapidly developed and tested in bacteria and then, with the addition of proper targeting
sequences, function in
plants.
While the Arabidopsis
sequence may speed up
plant science in general, the new MPK4 study will play into the hands of researchers interested in map kinase
signaling, says Giraudat.