Sentences with phrase «plated cells sorted»
Not exact matches
Single cells from this sorted population were then seeded into each well of a 96 - well plate by limiting dilution.
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Sorted human ILC3s (Lin - CD127 +) were cultured at 2,000 — 5,000 cells per well of a 96 well plate with irradiated (30 Gy) OP9 - DL1 feeders [70].
(C) Flow cytometry on cells plated at single cell density in 96 well plates from each sorted fraction.
On the other hand, if human material enter the plate of sorted cells while cDNA is being generated, human mRNA will convert to cDNA but with consistent bulk - like expression patterns in the different wells.
By necessity of the technology cells from each mouse and time point need to be sorted into individual microwell plates.
Sorted cells cultured in 48 - well plates for 17 days were washed once with PBS and fixed for 5 min.
CD4 + T cells sorted by immunomagnetic beads from splenocytes of animals tolerant to donor - type cardiac allografts were plated at 106 cells / well.