Sentences with phrase «pluripotency markers in»

These data show that pluripotency markers in the expanded clones are maintained at levels comparable to those in the parental line, ChiPSC18.

To our knowledge, this study is the first to identify a pluripotency marker in a heterogeneous population of human dermal fibroblasts, to isolate a subpopulation of cells that have a significantly increased propensity to reprogram to pluripotency and to identify a possible mechanism to explain this differential reprogramming.

Not exact matches

Whitehead Institute researchers have determined that the transcription factor Nanog, which plays a critical role in the self - renewal of embryonic stem cells, is expressed in a manner similar to other pluripotency markers.

To determine pluripotency and AcGFP1 knockout efficiency in gesicle - treated cells, cells were labeled with a fluorescently labeled antibody specific to the pluripotency marker SSEA - 4.

Pluripotency was maintained in all edited clonal lines, as evidenced by the persistent expression of the three pluripotePluripotency was maintained in all edited clonal lines, as evidenced by the persistent expression of the three pluripotencypluripotency markers.

RT - PCR analysis of RNA samples from EBs on days 3, 6 and 9 revealed a gradual decrease in expression of endogenous pluripotency markers such as Nanog, Rex1, Oct4 and Sox2 as compared to their expression in undifferentiated iPS cells (Figure 4B, upper panel).

Quantitative PCR analysis was performed to compare transcript levels of Venus with PrEn (Hex, Gata4, Gata6, Dab2, Sox7, Hnf4α, and Pdgfrα), pluripotency (Nanog, Klf4, Rex1, Stella, and Pou5f1) and other lineage (T, Fgf5, Eomes, Flk1, Mixl1, Cdx2, Sox1, Pax6, and Six3) markers in purified cell fractions.

Panel illustrates marker genes implicated in pluripotency of NSCs, with color bar reporting log2 normalized expression values (green / red indicates high / low relative expression).

Panel below illustrates marker genes implicated in pluripotency of NSCs, with color bar reporting log2 normalized expression values (green / red indicates high / low relative expression).

As seen in cells dissected from colonies, significant numbers of cells coexpressed lineage specific markers along with pluripotency genes.

In the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expressioIn the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expressioin the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expressioin cell surface marker expression.

Towards the center of the colony, ∼ 40 % of the cells had lost expression of all pluripotency markers, and we found a number of cells that were null - expressing no markers of pluripotency or lineage commitment, only the housekeeping gene cyclophilin a. However, many cells in this region still expressed Oct - 4.

Finally, within the fractionated cell populations, there was evidence for coexpression of lineage specific markers alongside of pluripotency genes, similar to lineage priming described first in hematopoietic stem cells [15].

They showed pluripotency in many ways, including their expression of pluripotency markers, such as OCT3 / 4, NANOG, SOX2, SSEA1, SSEA4, and AP in immunofluorescence assay, Oct4, Nanog, Sox2, Klf4 and cMyc in RT - PCR.

Oct - 4 is most consistently expressed of the pluripotency genes that we have studied, and it is switched off only in populations that have lost other measurable features of pluripotency, such as stem cell surface marker expression and the capacity for self - renewal.