Sentences with phrase «pooling of amplicons»
This allows for a one - step PCR reaction and pooling of amplicons from several different samples and targets (generated with other LymphoTrack Assays for the Illumina ® MiSeq ® instrument, sold separately), onto one MiSeq ® flow cell allowing for up to 24 samples per target to be analyzed in parallel in a single run.
https://www.invivoscribe.com/ Not exact matches
Calculating the concentration of PCR amplicons allows equal amplicon representation in the final pooled library that is loaded onto the MiSeq for sequencing.
Dilutions of the purified and pooled PCR amplicons were made and quantified using a synthetic oligonucleotide standard of known molarity [35], to approximate an optimal bead to template ratio for emulsion PCR.