The three lines are: (1) the beginning: the Khirthar transgression and the onset of neritic carbonate accumulation in the Bartonian Age (preceding onset of the Middle Eocene climatic optimum [MECO]-RRB-; (2) the midlife change (Bartonian - Priabonian transition): the shift from carbonate - rich to carbonate -
poor, higher - nutrient environments under estuarine circulation, causing widespread dysaerobia culminating in opaline silicas; and (3) the Eocene - Oligocene = Priabonian - Rupelian boundary and glaciation
during oxygen isotope event Oi - 1, with return of improved
ventilation in neritic environments and resumption of carbonate accumulation.