It was at this stage that highly purified
populations of endothelial cells could be obtained by adjusting the gating parameters to select only the most intensely labeled cells.
Not exact matches
The classical concepts
of angiogenesis consider the
endothelial cells within tumors as a rather passive
cell population that merely reacts in response to growth factors released by the tumor
cells.
The selection strategy targeted
cell populations expressing the inducible
endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular
endothelial cell lines from a number
of different organs.
Thus, our selection strategies, which were based on targeting
endothelial populations that expressed both
of these inducible glycoproteins, suggest that the
endothelial cell lines generated in this study are most likely from this microcirculatory region.
Once
endothelial cells are removed from a heterogeneous
population of cells, it is often difficult to obtain a sufficient number
of cells for experimental analysis.
Related to this, the discovery
of an increased
endothelial cell population in the periphery
of the human cornea has prompted an investigation for evidence
of the existence
of stem - like
cells in the
endothelial periphery.
The extensive apoptosis
of allogeneic corneal
endothelial cells by CD8 − T
cells from CD4 KO mice was perplexing, as this
cell population should not contain CD4 +
cells.
The postnatal adipose tissue contains a heterogeneous
population of cells which includes adult stem
cells (i.e., MSCs),
endothelial progenitor
cells, leukocytes,
endothelial cells, and vascular smooth muscle
cells.
This gave a mixed
population of cells with 20 % expressing CD34, a hematopoietic stem
cell marker, with some co-expression
of endothelial cell markers (CD31, Flk1, and VE - cadherin).
It is tantalizing to speculate that amiodarone HCl might also be toxic to other stem
cell populations that demonstrate increased ion channel expression relative to their differentiated derivatives, including mesenchymal stem
cells (MSCs) and
endothelial precursor
cells [36], thus expanding the utility
of the automated screening assay described here.
The low density culture protocol helps in generating larger number
of CD133 +
endothelial population from initially fewer CD133 +
endothelial progenitor
cells; EPCs (Figure S1).