Sentences with phrase «positive cells express»

(A) Venus positive cells express Oct4, but not Nanog.

To test the theory, Arlinghaus and colleagues first demonstrated that BCR - ABL positive cells expressed 24p3 and secreted it into the surrounding tissue, causing cell death.

Not exact matches

One clear positive element in the stem - cell debate for me was hearing the top researchers in biomedical science reinforce The Catechism of the Catholic Church (CCC 343: «Man is the summit of the Creator's work, as the inspired account expresses by clearly distinguishing the creation of man from that of other creatures»).

Unlike CD8 + DCs that express the cell surface protein CD205, CD8 — DCs, which are positive for the 33D1 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II.

The scientists already knew how to encourage mouse embryonic stem cells to express nestin, and they wondered if they could coax their nestin - positive cells to take on more characteristics of pancreas cells.

In mice expressing GFP under the control of the 5 - HT3A promoter, a subset of cells in the geniculate ganglion and nerve fibers in taste buds are GFP - positive.

Using a new TaqMan PCR - based technique, the researchers screened a number of cancer cell lines from various tissues, and discovered that tRNA halves were specifically expressed in large quantities in sex hormone - dependent cancers, i.e., estrogen receptor (ER)- positive breast cancer and androgen receptor (AR)- positive prostate cancer that are driven by the hormones estrogen and testosterone.

But scientists have discovered that misfolded proteins can have a positive side in yeast, helping cells navigate the dicey current of natural selection by expressing a variety of hidden genetic traits.

Like conventional T cells that express an αβ TCR, their development is initiated by positive selection at the CD4, CD8 DP developmental stage in the thymus.

Relative mRNA quantification via qPCR revealed that NG2 - positive cells were devoid of Pecam1 expression, but harboured Tek (Tie2) expressing cells (Fig. 4a).

In the thymus, expression of CD4 and CD8 varies during T cell maturation, such that cells initially do not express CD4 or CD8 (double negative [DN]-RRB- after arriving to the thymus, then express both CD4 and CD8 (double positive [DP]-RRB- after TCR rearrangement, and finally downregulate either CD4 or CD8 to generate mature single - positive cells that exit into the periphery.

On the other hand, there are many other tissues — notably, the kidney and articular cartilage — where p16Ink4a - expressing senescent cells appear to be a contributing factor to human and murine degenerative aging, but which were not evaluated in treated or control mice in this study, and it would be of interest to see the effects of ablation of p16Ink4a - positive senescent cells.

(6) Following this initial test of abrogating the early, age - related rise in p16Ink4a - expressing cell burden, the investigators probed the effects of leaving BubR1H / H; INK - ATTAC to undergo 5 months of rapid «premature aging» (and thus, to the attendant accumulation of high levels of p16Ink4a - positive cells and onset of «early - aging» phenotypes), and only then inducing ablation of senescent cells with the INK - ATTAC drug - activated system (see Figure 2 (g) below).

Positive clones were then differentiated using defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

The number and intensity of ROS - stained cells were captured with a GE InCell imager and the percentage of cells expressing ROS above a set threshold were determined; positive values show pairs of means that are significantly different.

Immunohistochemical staining of the automatically monitored colonies showed that these colonies were positive for Nanog and that SSEA1 was expressed in only a small proportion (3 %) of the cells (Figure 2f).

Moreover, cells expressing the V617F / Y931C double JAK2 mutant showed identical sensitivity to the inhibitors as cells expressing Y931C mutant alone, with a 20 to 50-fold increase in the IC50 (INCB018424 and CMP6, respectively) compared to V658F mutation - positive cells.

HV cells cultured under self - renewing conditions were subjected to flow cytometry to separate Venus positive and negative ES cell subpopulations and injected into Rosa26 LacZ expressing blastocysts within 1 h of purification.

Since V + cells were found abundantly in the SSEA - 1 positive population, we asked whether this population expressed other markers of the undifferentiated state.

Antibody staining for Nanog and Oct4, imaged alongside YFP / Venus fluorescence, indicated that while the Venus positive cells were also Oct4 positive, they expressed low levels of Nanog (Figure 3A).

Defined human cell line cores containing positive and negative protein expressing cell lines on the same slide

Venus positive cells experiencing low - level transcription at the Hex locus, but still expressing the ES cell markers SSEA - 1 and Oct4, show elevated levels of PrEn gene expression and reduced levels of early ICM markers such as Nanog.

We believe that a similar early precursor may exist to the PrEc lineage (Figure 9), and while we have no direct evidence for this, we did observe Oct4 positive cells that neither expressed Nanog nor the Venus transgene and there also appears a slight enrichment of early neural markers in the V − S + population (Figure 4).

Figure 2A shows that in the presence of the cytokine LIF, the majority of Venus - positive cells (70 %) were also SSEA - 1 positive (V+S +), while LIF withdrawal both increased the percentage of the population expressing high levels of the Venus transgene (mean level of fluorescence increases approximately 2-fold, Figure 2A) and led to a substantial increase in a second Venus positive population that is SSEA - 1 negative (V+S −).

In addition to the SSEA3 - positive and negative populations of cells, which represented the top 10 % and bottom 10 % of SSEA3 expressing cells respectively, we also included the intermediary SSEA3 - expressing cells, which represented the remaining 80 % of the total HUF1 cell population.

At 8 days these cells had a clear appearance (Fig. 8A), and rhodopsin - positive material was found within the CD68 - expressing cells (Fig. 8B).

Here we report that HB1.F3 NSCs can be engineered to express and secrete functional full - length HER2 - specific human immunoglobulin molecules that can selectively bind to and inhibit the proliferation of HER2 - positive breast carcinoma cells.

Immunofluorescence analysis of the two populations, following overnight adherence to exclude analysis of non-viable cells, revealed that > 97 % of the SSEA3 - positive population expressed detectable SSEA3 / 488 fluorescence and 0 % of the SSEA3 - negative population expressed detectable SSEA3 / 488 fluorescence (Figure 2C), demonstrating that the fluorescence activated cell sorting process can purify viable subpopulations of cells from a heterogeneous somatic population.

Andrews and coworkers [12] showed that ES cell cultures were comprised of populations that were positive or negative for cells expressing SSEA - 3.