Sentences with phrase «preparative cell sorts»
After 20 rounds of preparative cell sorts, the mutational machinery was stopped by inducing the excision of the floxed AID gene which is necessary for SHM [10].
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To avoid the appearance of AID negative clones, cells were cultured in the presence of 0.5 mg / ml of mycophenolic acid for three days following each preparative FACS sort.