Utilizes spontaneous and CRISPR / Cas9 generated mouse lines along with
primary cell culture models to look at the molecular intersection between the motor neuron and heart phenotypes in mouse models of Spinal Muscular Atrophy with Respiratory Distress (SMARD).
Not exact matches
He is also developing a robust and comprehensive panel of 3 - D
cell culture models from patient - derived
primary cells that can be used to characterize different disease phenotypes and investigate the chemo - response of
cells to novel or known drugs.
As bioinformatics approaches can only provide partial solutions, a functional analysis pipeline, based on
primary (fibroblast)
cell cultures, induced Pluripotent Stem
cells (iPS) or simple
model organisms, is truly essential.
We use a combination of conditional mouse
models,
primary cell culture (human and mouse), live microscopy, highthroughput genomics and proteomics approaches, and basic molecular biology.
Although this possibility has not yet attracted as much attention as the ideas of
cell replacement, personalized medicine and other more direct clinical applications, hESCs are expected to be superior to most commonly used
cell -
culture models of drug discovery which employ tumor - derived or immortalized
cell lines or
primary cell culture.