Sentences with phrase «primitive endoderm»

Analysis of Oct4 protein expression in differentiated Ara - C cultures (DACs) showed a slight increase of Oct4 protein by day four of aggregate formation (Figure 1C), consistent with previous reports examining Oct4 mRNA dynamics [22] and the finding that primitive endoderm differentiation is accompanied by a transient increase in Oct4 protein levels [23].

A more recent study showed overlapping expression of nanog with GATA - 6 and a Pdgfra reporter, markers of the primitive endoderm lineage, from the morula to the 64 cell stages [8], suggesting a gradual transition from a pluripotency program to a committed state.

Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst.

We show that the fraction of cells present within this state is influenced by factors that both promote and suppress primitive endoderm differentiation, but conditions that support ES cell self - renewal prevent their progression into differentiation and support an equilibrium between this state and at least one other that resembles the Nanog positive inner cell mass of the mammalian blastocysts.

One of these cells types, characterized by both the expression of the Venus transgene and the ES cells marker SSEA - 1 (V+S +), appears to represent an early step in primitive endoderm specification.

Here we use a transgenic allele designed to detect low level transcription in the primitive endoderm lineage as a tool to identify an immediate early endoderm - like ES cell state.

Not exact matches

For example, cells in the primitive streak can become either endoderm or one of two types of mesoderm.