Sentences with phrase «progenitor cell population»

Based on previous protocols [5, 6] they have now created a 3D protocol for chondrogenic lineage differentiation via the generation of a putative chondrogenic progenitor cell population, and have found that using this protocol C - iPSCs can be readily differentiated into cartilage in a manner comparable to that of mature chondrocytes [7].

AST - OPC1, an oligodendrocyte progenitor cell population derived from human embryonic stem cells, has been shown in preclinical testing in animals and in vitro to have three potentially reparative functions that address the complex pathologies observed in demyelination disorders, such as spinal cord injuries, and multiple neurodegenerative diseases, including multiple sclerosis and white matter stroke.

The researchers found that a specific stem / progenitor cell population was inhibited following radiation in mice missing Nrf2, and that alveolar type 2 cells in these mice were more likely to change into myofibroblasts — a cell type implicated in fibrosis.

Further challenges include the identification of signaling molecules that promote the generation of beneficial populations of astrocytes, identification of appropriate stem and / or progenitor cell populations that can be the source of such cells, and determination of whether there are situations in which it is more useful to transplant astrocytes themselves rather than to transplant stem or progenitor cells that might generate astrocytes in vivo in response to signals present in the host environment.

Zonari E, Desantis G, Petrillo C, Boccalatte FE, Lidonnici MR, Kajaste - Rudnitski A, Aiuti A, Ferrari G, Naldini L, Gentner B. Efficient ex vivo engineering and expansion of highly purified human hematopoietic stem and progenitor cell populations for gene therapy.

Not exact matches

For that, they quantified how 85 nanoparticles delivered DNA barcodes to eight cell populations in the spleen, and found that cell types derived from myeloid progenitors tended to be targeted by similar nanoparticles.

Researchers have identified a population of «progenitor» cells in the skin that are solely responsible for the generation and maintenance of touch - sensing Merkel cells.

In those cases, the NPCs often matured into adult kidney cells in a manner of days, leaving no steady population of progenitor cells to study.

When Kirkland took progenitor fat cells from human fats — subcutaneous, omental, and mesenteric — and cultured them, they retained their distinctive characteristics even after 40 population doublings.

The researchers also found that another population known as progenitor cells — differentiated daughter cells of stem cells — started to behave like stem cells: They began to live much longer than their usual lifespan of a few days, and they could also generate mini-intestines when grown outside of the body.

Using white fat tissue from liposuction or weight - loss surgery, «we might purify a population of these progenitor cells from an obese individual expressing C29 with high potential to become energy - dissipating cells,» she explains.

The postnatal adipose tissue contains a heterogeneous population of cells which includes adult stem cells (i.e., MSCs), endothelial progenitor cells, leukocytes, endothelial cells, and vascular smooth muscle cells.

Opening the way to accurately profile the chromatin states of in vivo stem cells, lineage progenitors and other scarce cell populations.

This approach not only enables us to get highly pure populations of DA progenitor cells (> 90 %), but it also increases our final yield of transplantable cells > 40 times when compared to previous embryoid body protocols starting with the same number of cells.

Discovered a series of signaling events beginning with the morphogen Sonic hedgehog (Shh) that are essential for guiding a population of late cardiac progenitor cells in the outflow tract of the heart.

These five types of stem cells are described in the Discovery database as a general dental pulp stem cell population, which includes the following stem cell types: dental pulp stem cell (DPSCs), stem cells from exfoliated deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), stem cells from apical papilla (SCAP), and dental follicle progenitor cells (DFPCs).

This approach reveals that a single population of progenitor cells maintains normal cell turnover of the epidermis and oesophageal epithelium.

This two - volume reference integrates this exciting area of biology, combining the prerequisites for a general understanding of adult and embryonic stem cells, the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations, as well as a presentation by the world's experts of what is currently known about each specific organ system.

The outcome of a given division is unpredictable, but in homeostasis the probabilities of producing two progenitor and two differentiating daughters are the same, so that on average, equal numbers of progenitors and differentiating cells are produced across whole population of progenitors.

Starting with transplants of human oligodendrocytes in the late 1980s [40], and more recently with populations of human oligodendrocyte progenitor cells isolated from the developing or adult CNS, or from human embryonic stem cells, it has been possible to generate extensive myelination upon transplantation into spinal cord injury or into congenital mouse models of hypomyelination [41]--[48].

As a first step towards determining whether human glial progenitor cells (hGPCs) can generate functionally distinct astrocyte populations, we exposed embryonic hGPCs isolated from spinal cords of 9.5 week old abortuses to BMP or CNTF.

Additional wells containing cells from the negative sort population were cultured as above and after 17 days, pebble - like clusters of small cells were excised and transferred to ENStem - A neural progenitor media (cat.

No significant differences were seen between the normoxic and hypoxic anoikis - resistant populations collected from MCF7 cells and a single significant gene change (PIP) was seen in T47D (Table 1) suggesting that the CSC - enriched population remains virtually unchanged following hypoxic culture and the increase in MFC, HFC, and tumor initiating cells is, therefore, due to expansion of the population, perhaps by increased symmetric self - renewal of the CSC or de-differentiation of early progenitor cells, rather than simply the acquisition of anoikis resistance in non-CSC.

This models depicts the ES cell as a highly plastic but nevertheless discrete and stable cellular entity, one that in turn gives rise through a massive switch in gene expression to discrete progenitor populations with more limited developmental potential.

The researchers suggest that a single expandable embryonic lung progenitor population with broader developmental competence may eventually be used as an alternative for region - restricted adult tissue stem cells in regenerative medicine.

Within the population of transcriptionally similar stem and progenitor cells, our analysis reveals considerable cell - to - cell differences in their probability to transition to another committed state.

The success of marker - based approaches for dissecting haematopoiesis in mouse and human is reliant on the presence of well - defined cell surface markers specific for diverse progenitor populations.

Our previous work has shown that the CD44 + and CD44 + α2β1 + prostate cancer cell populations are enriched in tumorigenic and metastatic CSCs, whereas the α2β1 + prostate cancer cells most likely mark fast - proliferating tumor progenitors (28, 29).

Satellite cells (SCs) are a heterogeneous population of stem and progenitor cells that have been demonstrated to play a pivotal role in skeletal muscle (SkM) regeneration.

The low density culture protocol helps in generating larger number of CD133 + endothelial population from initially fewer CD133 + endothelial progenitor cells; EPCs (Figure S1).

Considering that miR - 142 is highly expressed in human BCSCs, but weakly expressed or undetectable in the stem / progenitor population of the mammary epithelial cells, our result suggest that the upregulation of miR - 142 and its enhancement of the miR - 150 expression seem to be especially relevant in the breast tumor progression in vivo.