Sentences with phrase «promoter sequence upstream»

The shortest genomic region capable of rescuing forward and backward locomotion defects in unc - 7 mutants encompassed the coding region of unc - 7 and 1 kb of promoter sequence upstream of exon 2 (unc - 7 min; Figure 3B).

A shorter version of unc - 7 min lacked promoter sequences upstream of exon 2 (unc - 7S:: gfp; Figure 3C) and rescued forward but not backward locomotion, suggesting that promoter sequences in intron 3 (Punc - 7S; Figure 3C) drive a subset of UNC - 7S expression sufficient to rescue only forward movement.

Together these studies suggest that Punc - 7S drives expression of UNC - 7S in a subset of neurons (including AVA and AVB, but not motor neurons) that can effect rescue of forward locomotion, and promoter sequences upstream of exon 2 drive additional expression of UNC - 7S in motor neurons or possibly other neurons required to fully rescue all unc - 7 locomotory defects.

Not exact matches

When the promoter upstream of the GFP gene was activated, the RNA polymerase ran headfirst into the termination sequence, stopped reading the DNA, and didn't produce the fluorescent protein.

The vaccinia virus 13L early / intermediate promoter is cloned upstream of the nef coding sequences.

Conserved regions include the IR1 latency promoter (Wp), and one zone upstream of and two within BWRF1.IR1 is heterogeneous in 70 % of strains, and this heterogeneity arises from sequence exchange between strains as well as spontaneous mutation, with inter-strain recombination more common in tumour - derived viruses.

Cosmid F56B12 includes presumptive upstream promoter sequences and ends within intron 3.