Together these studies suggest that Punc - 7S drives expression of UNC - 7S in a subset of neurons (including AVA and AVB, but not motor neurons) that can effect rescue of forward locomotion, and
promoter sequences upstream of exon 2 drive additional expression of UNC - 7S in motor neurons or possibly other neurons required to fully rescue all unc - 7 locomotory defects.
A shorter version of unc - 7 min lacked
promoter sequences upstream of exon 2 (unc - 7S:: gfp; Figure 3C) and rescued forward but not backward locomotion, suggesting that promoter sequences in intron 3 (Punc - 7S; Figure 3C) drive a subset of UNC - 7S expression sufficient to rescue only forward movement.
The shortest genomic region capable of rescuing forward and backward locomotion defects in unc - 7 mutants encompassed the coding region of unc - 7 and 1 kb of
promoter sequence upstream of exon 2 (unc - 7 min; Figure 3B).
Not exact matches
When the
promoter upstream of the GFP gene was activated, the RNA polymerase ran headfirst into the termination
sequence, stopped reading the DNA, and didn't produce the fluorescent protein.
The vaccinia virus 13L early / intermediate
promoter is cloned
upstream of the nef coding
sequences.
Conserved regions include the IR1 latency
promoter (Wp), and one zone
upstream of and two within BWRF1.IR1 is heterogeneous in 70 % of strains, and this heterogeneity arises from
sequence exchange between strains as well as spontaneous mutation, with inter-strain recombination more common in tumour - derived viruses.
Cosmid F56B12 includes presumptive
upstream promoter sequences and ends within intron 3.