Sentences with phrase «protein copy numbers»

We aim to push its limits on all fronts to establish a technique which combines nanometre 3D resolution with maximum labelling efficiencies, absolute measurements of protein copy numbers, precise multi-colour measurements, high - throughput for large scale statistics and novel data analysis approaches, to address the vast array of exciting biological questions at the nanoscale, which are becoming accessible only now.

These new data suggest that transcriptome analysis can be used as a tool to predict the protein copy numbers per cell.

In the present study, the researchers have developed a mass spectrometry - based method that is sensitive and reproducible in order to measure, at steady - state conditions, absolute protein copy numbers across human tissues and cell lines and compared these levels with the corresponding mRNA levels using transcriptomics.

Not exact matches

The team integrated three, complementary gene sequencing approaches to look for mutations in tumor cells from SS patients: whole - genome sequencing in six subjects, sequencing of all protein - coding regions (exomes) in 66 subjects, and comparing variation in the number of copies of all genes across the genome in 80 subjects.

Those results are the same if the enhancers are intact but both copies of the gene itself are defective, indicating that the amount of protein determines the number of digits.

«Sometimes the criteria for HER2 positivity is a high level of HER2 protein, sometimes it's over-expression of the HER2 gene, and sometimes it's high HER2 copy number.

CCG uses high - throughput techniques to identify and study mutations, large rearrangements of the genome, increases and decreases in DNA copy number, chemical modifications to DNA, and changes in the expression of RNA and proteins.

It is always good to keep in mind that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper promoter and growth conditions.

This section invites manuscripts describing (a) Linkage, association, substitution or positional mapping and epigenetic studies in any species; (b) Validation studies of candidate genes using genetically - engineered mutant model organisms; (c) Studies focused on epistatis and gene - environment interactions; (d) Analysis of the functional implications of genomic sequence variation and aim to attach physiological or pharmacogenomic relevance to alterations in genes or proteins; (e) Studies of DNA copy number variants, non-coding RNA, genome deletions, insertions, duplications and other single nucleotide polymorphisms and their relevance to physiology or pharmacology in humans or model organisms, in vitro or in vivo; and (f) Theoretical approaches to analysis of sequence variation.

High gene copy number variation (CNV) among membrane - bound transporters, a class of proteins previously implicated in drug resistance, was found for the most highly differentially expressed genes.

However, being a complex multistep process, cancer cytogenetics are broadened to «cytogenomics,» with complementary resources on: general databases (nucleic acid and protein sequences databases; cartography browsers: GenBank, RefSeq, UCSC, Ensembl, UniProtKB, and Entrez Gene), cancer genomic portals associated with recent international integrated programs, such as TCGA or ICGC, other fusion genes databases, array CGH databases, copy number variation databases, and mutation databases.

Evaluation of SMN protein, transcript, and copy number in the biomarkers for spinal muscular atrophy (BforSMA) clinical study.