Sentences with phrase «protein labeling»

The rats gave the researchers a potential new window into how these circuits form in human brains, where alcohol - linked neurons are harder to identify without the use of protein labels.

The Human Protein Atlas displays high resolution, multicolor images of proteins labeled with immunofluorescence at the single cell level.

HeLa cells, with proteins labeled in blue and DNA in red, are responsible for over 20 percent of the cell line contaminations.

Green fluorescent protein labeling allowed them to see the early development pattern and show that lncND, which ordinarily is not present in mice — lncND is present only in some primates including humans — had a functional effect on development.

To date, this approach «is still the only generalizable small - tag protein labeling method that works inside living cells.»

For example, peas are healthy for dogs but because of their high protein content, high amounts in Zignature's recipes can boost the total protein labelled on the dog food.

We are developing proteomics - based methodologies, including selective protein labeling, multi-dimensional electrophoresis, and mass spectrometry techniques, to identify the products of proteolysis in vivo.

Purified MECA - 79 was labeled with the Alexa Fluor 488 Protein Labeling Kit (Thermo Fisher Scientific, Waltham, MA).

We have established a large repertoire of molecular tools for surface modification and protein labeling based on tris - NTA (3).

Optimize protocols for culturing and maintaining Hela cells, identifying and characterizing proteins, enrichment of integral membrane proteins from mammalian tissue, biotinylation and isolation of mammalian cell surface proteins, and isotope metabolic protein labeling, quantization of protein expression levels from differentially treated cell populations.

Basic techniques: SDS - PAGE gels, native gels, protein concentration by Bradford, protein labeling with fluorescent markers (Alexa etc)

Saturated depletion enhances the resolution of SIM images by taking advantage of fluorescent protein labels that can be switched on and off with light.

«This is the first time this method of protein labeling has been used to measure the act of translation of multiple proteins over space and time in a quantitative way,» says Eberwine.

In humans, there are about 100 proteins associated with ubiquitination, the process by which a protein labelled with ubiquitin is removed from the cell by specific cell machinery known as the proteosome.

At her lab at MIT, where she is now an Associate Professor with Tenure, Dr. Ting and her colleagues took an unprecedented approach to solve the problem of site - specific protein labeling: to use reengineered natural enzymes.

In 2002, an offer to join the faculty of MIT's Chemistry Department drew her back to the East Coast, where she was able to begin tackling the problem that had «repeatedly stymied» her throughout her PhD and postdoc years: site - specific protein labeling — how to label a particular protein with a fluorescent tag or other «chemical probe» to reveal information about that protein's function.

Protein labeling and biotinylation of peptides during spot synthesis using biotin p - nitrophenyl ester (biotin - ONp).