Sentences with phrase «protein liquid chromatography»

ApoC - III ASO treatment reduced TG levels in chylomicrons, VLDL, and remnant particles in all of the mutants as measured by size - exclusion fast protein liquid chromatography (FPLC)(Figure 2, A — E), but had no effect in Ldlr — / — Lrp1fl / fl Alb - Cre + mice (Figure 2F).

The researchers combined protein purification techniques including fast protein liquid chromatography, with modern metagenomics, metaproteomic and associated bioinformatics analyses.

Not exact matches

The process involving liquid chromatography - electrospray ionization - tandem mass spectrometry relies on a vital step called «charge derivatization» or using a permanently charged reagent to selectively trap the estrogens and isolate them from the lipids and proteins that could interfere with estrogen detection, Schug said.

Methods: The researchers used capillary liquid chromatography - mass spectrometry to extensively characterize proteins present in the Salmonella wild - type and mutant strains.

Magno Junqueira (Shevchenko, MPG)-- «Systematic characterization of mammalian protein complexes by shotgun liquid chromatography tandem mass spectrometry» (2009)

In MudPIT, a complex mixture of proteins and peptides are separated using 2 - D liquid chromatography, and then the separate proteins are digested (chopped into smaller «peptide» pieces with enzymes) and subjected to the sensitive technique of mass spectrometry (which identifies the components based on their masses).

They used liquid chromatography and tandem mass spectroscopy to identify proteins in the complex.

Monoclonal antibodies are large, complicated proteins that must then be separated and purified, first in centrifuges and after that with liquid chromatography.

Brains that have been voxelated into ~ 700 cubes will be prepared using automated microscale sample processing and analyzed using high - throughput reversed - phase liquid chromatography coupled online with high - resolution mass spectrometry to obtain both the spatial localization and relative abundance of brain proteins.

Methods: The researchers used capillary liquid chromatography - mass spectrometry analyses to analyze the proteomes of Salmonella mutant strains lacking specific RNA - binding proteins that mediate the translation of RNA into protein (post-transcriptional control) and the parent strain grown under four different conditions.

Liquid chromatography coupled to mass spectrometry (LC - MS) is becoming the method of choice for examining complex biological specimens, that contain hundreds to thousands of proteins [9], such as CSF [10].

Proteins were expressed, purified, and characterized via a microscreening protocol, which incorporates protein quantification, liquid chromatography mass spectrometry and analytical size exclusion chromatography (AnSEC) to evaluate suitability of the protein products for X-ray crystallography.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign