Sentences with phrase «protein lysates»

We thank D. Dorward and O. Steele - Mortimer for help with confocal microscopy; S. Raffel and G. Sylva for sequencing; T. Hackstadt for assistance with photography; M. Schrumpf for supplying protein lysates and E. coli strains; R. Gilmore, M. L. Mbow, and O. Steele - Mortimer for providing antibodies; K. Caughey, G. Hettrick, and A. Mora for graphic support; and F. DeLeo, T. Hackstadt, and S. Priola for critical review of the manuscript.

Western blot analysis of antibody specificity has been done using a routine sample setup composed of IgG / HSA - depleted human plasma and protein lysates from a limited number of human tissues and cell lines.

Normalized protein lysates were subjected to SDS - PAGE, transferred to nitrocellulose, and immunoblotted with primary Abs from Cell Signaling Technology (Danvers, MA) at concentrations recommended by the manufacturer.

Not exact matches

Using the kits, you can quickly and easily label proteins in lysates or purified proteins with near - infrared CF ® dyes before electrophoresis and blotting.

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

The research, performed using recombinant protein from cell lysate isolated in vitro, also validates a methodology for characterizing large, multivalent membrane proteins in general.

Lysates were immunoblotted for protein levels of (A) phosphorylated ERK (pERK), total ERK (tERK), and β - actin or (B) phosphorylated IκBα (p - IκBα), total IκBα (IκBα), and β - actin.

Cell lysates were incubated with protein G sepharose beads (GE Healthcare) and 3 μg Tie2 antibody (Millipore, clone Ab33, # 05 - 584) overnight at 4 °C on a rotator.

The Clinical Biomarkers Facility offers services for high - throughput and highly specific analyses of protein biomarker candidates in body fluids such as plasma, serum, cerebrospinal fluids etc. and cell and tissue lysates using molecular tools such as proximity extension and proximity ligation technologies (PEA and PLA) providing assays with high specificity and sensitivity in complex biological matrices.

Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).

Lysates from these two cell populations were fractionated on the basis of the subcellular localization using the ProteoExtract Kit, and 40 μg of proteins was loaded for Western blotting.

All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».

The Assay Designs ® SMN (human) Enzyme Immunometric Assay (EIA) kit is a complete kit for the quantitative determination of SMN protein in cell lysates of human origin.

The PGE2 concentration was corrected by the protein concentration of the cell lysates and presented as pg / μg protein.

Whole - cell lysates from B. burgdorferi and from Escherichia coli expressing recombinant P39 [BmpA (Borrelia membrane protein A)-RSB-(19) or recombinant His - tagged OspC (20) were prepared as described in ref.

Protein concentrations of the lysates were determined using the BioRad DC Protein Assay (Hercules, CA).

We validated FFId based on a public benchmark dataset, comprising a yeast cell lysate spiked with protein standards that provide a known ground - truth.

Unmatched performance — high transfection efficiency & cell viability in primary cells Fully scalable — from R&D to the clinic without reoptimization Safe — non-viral cell engineering in closed, sterile, computer - controlled environment Cell loading flexibility — mRNA, gRNA, siRNA, DNA, proteins, cell lysates, and small molecules into autologous or allogenic immune, stem / progenitor, or somatic cells Regulatory ease — Master File designation with the CBER Division of the U.S. FDA, cleared by NIH's RAC and Health Canada, cGMP - compliant, and CE - marked

The antibody was incubated under agitation with Protein G beads for 10 min, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation.

Finally, total radioactivity and total cellular protein of the lysate were determined.

Lysates were pre-cleared with Protein A-agarose beads blocked with 2.5 mg / mL sonicated salmon sperm DNA (Sigma - Aldrich) and 0.1 % bovine serum albumin (BSA, Santa Cruz Biotechnology).

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

Water, Bifida Ferment Lysate, Glycereth - 26, Juniperus Chinensis Xylem Extract, Sorbus Commixta Extract, Bis - PEG - 18 Methyl Ether Dimethyl Silane, Butylene Glycol, Niacinamide, Glycerin, Betaine, Chamomilla Recutita (Matricaria) Flower Extract, Disodium EDTA, Silica, Tripeptide - 1, Tripeptide - 10 Citrulline, Hydrolyzed Soy Protein, Hydrolyzed Wheat Protein, Ceteth - 3, Ceteth - 5, PEG - 5 Rapeseed Sterol, Caprylic / Capric Triglyceride, Limnanthes Alba (Meadowfoam) Seed Oil, Sea Water, Portulaca Oleracea Extract, Chamomile Flower Extract, Ethyl Hexanediol, Cyclopentasiloxane, Dimethicone, Glyceryl Polyacrylate, PEG - 11 Methyl Ether Dimethicone, PEG - 40 Hydrogenated Castor Oil, PPG -26-Buteth-26, Polysorbate 20.

Ingredients Cyclopentasiloxane, Dimethicone Crosspolymer, Polysilicone - 11, C12 - 15 Alkyl Benzoate, Polyglyceryl - 4 Isostearate, Cetyl PEG / PPG - 10 / 1Dimethicone, Hexyl Laurate, Tetrahexyldecyl Ascorbate, Tocopheryl Acetate, Retinyl Palmitate, Silica, Milk Protein, Bifida Ferment Lysate, Retinol, Caprylic / Capric Triglyceride, Butylene Glycol, Xanthan Gum, Cyclodextrin Laurate, Phenoxyethanol, Water, Mica, Titanium Dioxide (CI 77891).

The Heart for Companion Animal Research at Colorado State College has shown that cats vaccinated with FVRCP vaccines grown on Crandell - Rees Feline Kidney (CRFK) cell traces can develop antibodies to renal (kidney) proteins, and that cats hypersensitized to CRFK cell lysates can develop interstitial nephritis.