Sentences with phrase «protein microarray»

CDI has produced the ZikaProt ZIKV / DENV protein microarray which contains the proteomes of Asian and African strains of Zika as well as dengue serotypes 1 and 2.

SCIENTIST: Well, we're still in the theoretical stage, but if we can miniaturize the circuitry, we can improve the printing of certain protein microarrays.

Together these costs have prevented the widespread use of protein microarrays despite their enormous potential.

To produce standard protein microarrays small amounts of proteins are printed to a glass slide and chemically fixed in each spot where they are then available for experiments.

Protein microarrays allow the investigation of thousands of proteins in a single experiment.

[NEWS, 4 June 2013] Protein microarrays, representing more than one third of all human proteins, provide a unique possibility to study complex autoimmune diseases, such as multiple sclerosis (MS).

Bae N, Viviano M, Su X, Lv J, Cheng D, Sagum C, Castellano S, Bai X, Johnson C, Khalil MI, Shen J, Chen K, Li H, Sbardella G, Bedford MT. (2017) Developing Spindlin1 small - molecule inhibitors by using protein microarrays.

The use of protein microarrays to evaluate antibody cross-reactivity will likely set new quality standards ahead of published research, grants awards and reagent commercialization.

Instrumentation and know - how is available for generating customized spotted protein microarrays as well as measurement and analysis of commercially available protein microarrays.

Not exact matches

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

Therefore, before publication, large data sets (including microarray data, protein or DNA sequences, atomic coordinates or electron microscopy maps for molecular and macromolecular structures, and climate data) must be deposited in an approved database and an accession number or a specific access address must be included in the published paper.

Statistician Ker - Chau Li has found a cubical generalization of the correlation function, which he has used to detect, automatically, candidates for three - way interactions of proteins using microarray data.

Subjecting N. gonorrhoeae to the phenotypic microarray screening method for the first time, Sikora's team focused on seven proteins from the bacteria's cell envelope, which consists of the outer membrane, the cell wall and the inner membrane.

We have compared the transcriptome in blood leukocytes, liver, and brain of humans, chimpanzees, orangutans, and macaques using microarrays, as well as protein expression patterns of humans and chimpanzees using two - dimensional gel electrophoresis.

The Monash BDI researchers worked in collaboration with Canadian - based company Kinexus, and used an antibody microarray to simultaneously investigate hundreds of factors involved in cell signalling that were modulated by HCV replication, including human protein kinases.

For expression analysis in mice, we used microarray data as described above to select two internal control genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).

Antibody microarrays are powerful tools for simultaneous detection of hundreds or thousands of blood - borne protein biomarkers at once, potentially allowing accurate fingerprinting and improved diagnostics of complex pathophysiological states such as cancers, neurodegenerative diseases and autoimmune conditions.

We are now expanding this facility to not only include state - of - the - art protein domain microarrays, but also to allow the further characterization of these protein - protein interactions in cells.

Contact Mark Bedford ([email protected]) for experiments involving the use of protein domain microarrays, and contact Shawn Bratton ([email protected]) for experiments involving the biophysical analysis of protein complexes.

Services provided by EMBL Heidelberg include the Core Facilities which provide internal users, collaborators and visitors with access to excellent facilities for high - throughput analysis including microarrays, protein production, mass spectrometry, DNA sequencing, advanced light and electron microscopes and chemical genomics.

For microarray and qRT — PCR measurements (i.e., all molecular measurements except those for HIF - 1α message and protein), animals were sacrificed by decapitation without anesthesia either immediately following 3 h of hypoxia or following a 24 h post-hypoxia recovery period in normal air.

Protein subcellular localization profiling of breast cancer cells by dissociable antibody microarray staining.

Clinical Diagnostics The platform will provide service and support in order to promote and adapt the use of high throughput techniques, such as next - generation sequencing, microarray - based analyses and multiplex protein analysis, in routine diagnostics.

The completion of a draft genome sequence for the microorganism Methanosarcina barkeri has enabled use of high - throughput technologies such as microarrays and mass spectrometry of proteins to detect global changes that occur in response to experimental treatments.

The tissue microarray method used within the human protein atlas enabled the global mapping of proteins in the human body, including the brain.

Proteomic analysis to profile protein abundance resulted in the identification and relative quantification for 912 proteins with two or more unique peptides and 86 proteins with significant abundance changes after treatment with the neurotoxins, while microarray analyses to profile gene expression revealed 181 genes with significant changes in mRNA after treatment.

CDI Laboratories offers the Mycobacterium Tuberculosis Proteome Microarray featuring 4,262 full - length recombinant proteins encompassing > 95 % of the MTB proteome — a powerful, high - throughput analytical tool.

Microarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray (19,394 human proteins) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarrMicroarray Analysis: Test antibody specificity was evaulated using CDI HuProt ™ Human Proteome Microarray (19,394 human proteins) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarrMicroarray (19,394 human proteins) and subseqently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarraymicroarray images.

To gain a deeper understanding of the cellular response of the two neurotoxins, the researchers applied global quantitative proteomics at the U.S. Department of Energy's national user facility — the Environmental Molecular Sciences Laboratory — and microarrays at UCLA to assay the protein and gene levels, respectively, for both neurotoxins.

Other proteins can't be put on a microarray at all because they are part of a cell membrane and need to be attached to their membranes to function.

Using the HuProt microarray array, the binding of fluorescently labeled or biotinylated sample proteins to proteins on the HuProt human proteome microarray — the largest collection of human proteins in the world — can be analyzed simultaneously.

We used custom microarrays to capture and Illumina sequence 6 Mb of targeted intronic DNA, and combined this dataset with published protein coding sequences derived from transcriptome sequencing [28]--[29].

Microarray profiling identified STAT6 as one of the proteins whose expression was increased in apoptosis - resistant cells (Gagarin et al., 2005).

The microarray allows the specificity of fluorescently labeled, biotinylated or radiolabeled molecules to be assayed against the largest collection of human proteins in the world.

We can make cell microarrays that contain a very large percentage of the human genome without being limited by the protein's properties,» says Sabatini.

HuProt Cross-Section: microarray probed with a serum Ig sample and fluorescently - labeled secondary antibodies to the Ig and common epitopes of the arrayed proteins.

This is accomplished by combining high - throughput generation of affinity - purified antibodies with protein profiling in a multitude of tissues and cells assembled in tissue microarrays.

In addition to analysis using the full HuProt microarray, CDI can custom print microarrays containing up to 14 separate subarrays - each containing only proteins of interest as determined during the discovery phase.

Other activities (e.g. to provide recent information about recombinant DNA experiments, to support DNA / protein sequencing and microarray experiments, and so on..)

• Organized workflow directed towards bioassay design using Protein and DNA signatures on microarrays to test efficacy of detecting bio-threats from samples that included challenged and naturally occurring outbreaks.

Liquid handling automation, optical fluorescence detection, DNA / RNA / Protein microfluidics and microarray applications.