The scientists developed a system that facilitates the separation and isolation of the pseudopodium (PD, or leading part of the cell) and the cell body (CB) compartments of chemotaxing cells for
proteomic analysis using microporous filters.
Not exact matches
Using proteomics, microscopic
analysis, and functional assays, the group provided definitive evidence that membralin functions as part of the ERAD system.
Next, the contents of the normal and stress - exposed exosomes were characterized and compared
using various molecular and cellular biologic techniques,
proteomics analysis followed by bioinformatics
analyses.
The researchers
used a multitude of screening tools in this study to analyze the molecular components in the blood, including mass spectrometry - based quantitative
proteomic, metabolomic and hematological
analyses of blood cells.
Using mass spectrometry and bioinformatic
analysis in close cooperation with the Core Facility
Proteomics of the Helmholtz Zentrum München and the Bioinformatics and Computational Biology Department of the Technische Universität München, the researchers succeeded in precisely identifying a multitude of surface proteins.
Mass spectrometry was also
used for
analysis of proteins, an approach called
proteomics, led by Brook Nunn at the University of Washington.
BDNF induces widespread changes in synaptic protein content and up - regulates components of the translation machinery: an
analysis using high - throughput
proteomics.
PNNL scientists Dr. Mary Lipton and Dr. Michael Wilkins carried out the
proteomic analyses of the samples,
using capabilities at EMSL, a national user facility located at PNNL.
These comprehensive CSF
proteomics datasets were then
used to create an accurate mass and time (AMT) tag database for subsequent label - free quantitative
analysis of individual subject CSF samples.
The researchers
used Western Blot
analysis to confirm the
proteomic results.