The goals of the project are as follows: (1) to shotgun sequence the C. murisgenome to 8X coverage; (2) to sequence 20,000 clones from a C. muris cDNA library to
provide gene prediction training and verification data; and (3) to manually annotate the C. muris genome sequence in comparison with the genome sequence data for C. parvum and C. hominis.
The projects will
provide training in: current molecular biology techniques (including RNA interference, genetic transformation, analyses of
gene expression); cell biology techniques (cell culturing, cell transfections, imaging); protein biochemistry (2D electrophoresis, protein functional analyses); and bioinformatics (structure
predictions, phylogenetic analyses; molecular interactions).