The cells were then incubated with ab7291 at 1μl / ml and ab6046 at 1µg / ml overnight at +4 °C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor ® 488 (ab150117) at 2 μg / ml (shown in green) and anti-rabbit AlexaFluor ® 594 (ab150088) at 2 μg / ml (shown in
pseudo color red).