Sentences with phrase «purified cell fractions»
Quantitative PCR analysis was performed to compare transcript levels of Venus with PrEn (Hex, Gata4, Gata6, Dab2, Sox7, Hnf4α, and Pdgfrα), pluripotency (Nanog, Klf4, Rex1, Stella, and Pou5f1) and other lineage (T, Fgf5, Eomes, Flk1, Mixl1, Cdx2, Sox1, Pax6, and Six3) markers in purified cell fractions.
http://journals.plos.org/ Not exact matches
Initially we injected purified fractions of HV ES cells into Rosa26 blastocysts that constitutively express β - galactosidase (β - gal) and examined embryos at 9.5 dpc for ES cell (β - gal negative) contribution (Table S4 and Figure S5).
When purified V+S + or V − S + cells are placed back into self - renewing conditions, individual cells from purified fractions of either cell type regenerate their counterparts.