Sentences with phrase «pyrosequencing of»

Methodology / Principal Findings: Fecal samples from healthy dogs (n = 32), dogs with acute non-hemorrhagic diarrhea (NHD; n = 12), dogs with acute hemorrhagic diarrhea (AHD; n = 13), and dogs with active (n = 9) and therapeutically controlled idiopathic IBD (n = 10) were analyzed by 454 - pyrosequencing of the 16S rRNA gene and qPCR assays.

Pyrosequencing of 16S rRNA genes and temporal temperature gradient electrophoresis (TTGE) profiles in the colonic digesta showed that rats fed RS had altered microbiota compositions due to blooms of Bacteroidetes and Actinobacteria.

Here we use pyrosequencing of the bacterial 16S rRNA gene to quantify bacterial taxa [21].

Microbial DNA from colon specimens were isolated and analyzed by use of 454 pyrosequencing of 16S RNA genes.

Not exact matches

One commercially available alternative is pyrosequencing, which also detects nucleotides as they are added to a single strand of DNA, but it can lead to errors because it is harder to differentiate between a single base and a whole stretch of identical bases next to each other, says Elaine Mardis, a geneticist at Washington University in St Louis, US.

Pbo's group used a technique developed by Branford, Conn. - based biotech company 454 Life Sciences called pyrosequencing, which takes advantage of the fact that ancient DNA is often fragmented.

The team employed a new, massively parallel technique called pyrosequencing, which uses pulses of light to read the sequence of thousands of bases at once.

The recent introduction of the pyrosequencing - based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS.

The facility's infrastructure includes a pyrosequencing genetic analyser (PyroMark ID MA) capable of quick and efficient SNP analysis of as many as 480 samples per day, in addition to all the necessary equipment for a more conventional microsatellite genotypic analysis.

Work scheme to isolate the different microorganisms found in hydrogen - producing reactors: A study of effectiveness by pyrosequencing analysis — Laura Fuentes — Journal of Applied Microbiology

A Sanger / pyrosequencing hybrid approach for the generation of high - quality draft assemblies of...

A Sanger / pyrosequencing hybrid approach for the generation of high - quality draft assemblies of marine microbial genomes.

Amplicons were sequenced on a multiplexed 454 - FLX - Titanium pyrosequencing run at the Human Genome Sequencing center at Baylor College of Medicine.

One question in analyzing microbial communities by 16S rRNA gene pyrosequencing centers on whether the amplification and sequencing methods result in recovery of sequences in proportion to their representation in the original community [24].

Because < 20 % of all gastrointestinal bacteria can be cultured using conventional methodologies, we performed massively parallel pyrosequencing to further assess the qualitative changes in the intestinal microbiome following alcohol exposure.

Mostafa Ronaghi, Ph.D., Stanford Genome Technology Center, Palo Alto, Calif. $ 1.8 million (3 years) «Pyrosequencing Array for DNA Sequencing» The principal investigator of this team is an inventor of pyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminPyrosequencing Array for DNA Sequencing» The principal investigator of this team is an inventor of pyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminpyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminescent signal.

Comparative Analysis of the Gut Bacterial Community of Four Anastrepha Fruit Flies (Diptera: Tephritidae) Based on Pyrosequencing — Carmen Ventura — Current Microbiology

Barcoded Pyrosequencing Reveals a Shift in the Bacterial Community in the Rhizosphere and Rhizoplane of Rehmannia glutinosa under Consecutive Monoculture — Linkun Wu — International Journal of Molecular Sciences

The exact number of bases in a homopolymeric stretch becomes difficult to resolve by pyrosequencing, and as a result, 454 sequencing tends to overcall or undercall bases in these regions.

Unfortunately, indels are both the strength and the weakness of 454 data — due to the underlying pyrosequencing, homopolymeric regions are often under - or over-called, resulting in numerous false positives.

Services include: consultation with investigators to chose the best method of analysis; sample preparation, assay design and processing of the most used methods to study DNA methylation, both gene - specific and genome - wide (PyroMeth, RRBS and Methyl - Seq); and assessment of global DNA methylation by pyrosequencing methylation analysis of repetitive elements (LINE - 1 and Alu repeats).

These studies, however, were based on comparing Life Technologies» SOLiD and Illumina's GAII with pyrosequencing or Sanger sequencing [8], [9], [10], the costs of which are prohibitive for whole - genome sequencing studies in mammals.

We investigated the distal gut bacterial communities of three healthy humans before and after treatment with ciprofloxacin, obtaining more than 7,000 full - length rRNA sequences and over 900,000 pyrosequencing reads from two hypervariable regions of the rRNA gene.

Computer simulations by Liu et al. [43], Wang et al. [41], and Sundquist et al. [47] have indicated that pyrosequencing tags from different regions of the gene will vary in their utility for the distinct tasks of revealing microbial diversity and performing taxonomic classification, both of which contribute to making informative comparisons between complex microbial communities.

454 pyrosequencing reveals a shift in fecal microbiota of healthy adult men consuming polydextrose or soluble corn fiber.

The researchers then analyzed the types of bacteria present in each sample using a technique called 454 pyrosequencing.

Molecular assessment of the fecal microbiota in healthy cats and dogs before and during supplementation with fructo - oligosaccharides (FOS) and inulin using high - throughput 454 - pyrosequencing

B. Correlation of the fold differences between CPA and control obtained by Q - MeDIP and by microarray for the 20 amplicons analyzed in A. C. DNA methylation differences (%) between the groups in the GRM5, ITGB6, OR13C8 and IL - 31 genes validated by pyrosequencing.