Motivation: Single Molecule Real - Time (SMRT) sequencing technology and Oxford Nanopore technologies (ONT) produce reads over 10kbp in length, which have enabled high -
quality genome assembly at an affordable cost.
We've made it possible for any researcher to get a high -
quality genome assembly for their plant or animal of interest.
While the technology was used with the goal of attaining very high
quality genome assemblies, DOE JGI scientists have now additionally taken advantage of this sequencing platform to explore epigenetic (5mC, 6mA) modifications.
Moreover, we demonstrated that although the Oxford Nanopore technology is a relatively new sequencing technology, currently with a high error rate, it is already useful in the generation of high -
quality genome assemblies.
Ed's lab has recently pioneered a new approach to de novo genome assembly for the company Dovetail Genomics, making high
quality genome assemblies faster to produce and affordable for non-model organisms.
It must also be clearly stated that sufficient genetic material is available from the study subjects such that a range of sequencing technologies can be used in order to produce high
quality genome assemblies.
Not exact matches
The performance of our workflow was assessed on vertebrate
genome assemblies of various
qualities (platypus, pig, horse, dog, mouse and human).
«For chloroplast
genome recovery from total DNA sequence data, the deliberate identification of reads that represent chloroplast DNA inserts into the nuclear
genome allowed us to attain a higher -
quality chloroplast
genome assembly in a time - and cost - effective way,» Garaycochea explains.
The system is designed for projects such as rapid and cost - effective generation of high -
quality, whole -
genome de novo
assemblies.
James Giovannoni generated the gene expression data through RNA - sequencing and Lukas Mueller provided additional analysis to confirm the
quality of the
genome assembly.
A Sanger / pyrosequencing hybrid approach for the generation of high -
quality draft
assemblies of marine microbial
genomes.
We have recently developed hybrid
assembly methods that use a combination of short and long reads to produce remarkably high -
quality assemblies from whole -
genome shotgun data.
«Starting 2007, the first single - cell
genomes from environmental cells appeared in public databases and they are draft
assemblies with fluctuations in the data
quality.
The new high -
quality PcyM
assembly has an improved representation of the subtelomeric regions of the
genome, which now encompass nearly 40 % of the
genome sequence.
The technology allows scientists to go beyond fragmented draft
genomes and generate the most comprehensive and high -
quality de novo
assemblies.
High -
quality de novo
genome assembly of the Dekkera bruxellensis UMY321 yeast isolate using Nanopore MinION sequencing