Sentences with phrase «quantify gene expression»

We successfully established sequencing - based protocols to quantify gene expression of thousands of single cells.

A key question is how the variety of available protocols compare in terms of their ability to detect and accurately quantify gene expression.

Developing novel tools to quantify gene expression in single cells As it has become increasingly apparent that gene expression in individual cells deviates significantly from the average behavior of cell populations, new methods that provide accurate integer counts of mRNA copy numbers in individual cells are needed.

From the quantified gene expression / abundance values we can compare the controbuting sources in each individual cell, stratified by plate to see if there are any trends.

Not exact matches

In addition, the researchers have quantified the preservation level of this gene expression between humans and mice.

«We used the Allen Human Brain Atlas data to quantify how consistent the patterns of expression for various genes are across human brains, and to determine the importance of the most consistent and reproducible genes for brain function.»

However, that assay doesn't pinpoint the moment of activation or provide a straightforward way to quantify the connections between microglial activation and gene expression patterns.

They then used next generation sequencing — a state - of - the - art method to rapidly measure gene expression — to sequence and quantify the thousands of genes that are expressed in hair cells, in comparison with other cells in the ear.

The problem chiefly involves antibodies, a class of proteins scientists have used for generations for everything from quantifying receptor expression to purifying critical gene products.

Relative expression was quantified for Bsl1 and SvDwf4 genes in roots, sheaths, and 4th leaves from A10.1 seedlings at 20 DAS and inflorescence primordia at 18 DAS.

Panels B — D. mRNA expression analysis of genes involved in glycogen metabolism (Panel B), gluconeogenesis (Panel C), and insulin signaling (Panel D), as quantified by transcriptome analysis, was performed in enhanced hiPS - HEP cells from C12, C18, and C22 on Day 12 post-thawing (n = 2 batches per cell line) and compared to gene expression measurements performed on hphep cells on Day 1 post-thawing (n = 3 donors).

Since the expression of genes representing oxidative phosphorylation is decreased in adipose tissue of T2D twins (Table 2 and Fig. 1A), we quantified mtDNA content to follow up these results.

These studies will investigate the consequences of ApoF knockdown on cholesterol disposition in an animal model, quantify ApoF levels in human subjects and identify lipoprotein properties that cause ApoF to bind and subsequently inhibit cholesteryl ester transfer protein activity, and investigate the molecular mechanisms controlling ApoF gene expression.

RNA will be quantified by fluorimetry, retrotranscribed and then sequenced using Ion AmpliSeq ™ Transcriptome Human Gene Expression Kit on the «Ion Proton ™» (Thermo Fisher) sequencing platform.

The inflammatory effects of insufficient sleep were quantified in a study32 that deprived participants of sleep (just under six hours) for one week resulting in expression of genes associated with oxidative stress and inflammation.