Sentences with phrase «rrna marker genes»

In addition both constructs contain the constitutive Ubi promoter and nos T terminator, the bar gene conferring herbicide tolerance and may contain further sequences of bacterial origin including the neomycin phosphotransferase (nptI) selectable marker gene.

Those that survived developed into embryos, 28 of which were implanted into 11 cows; three gave birth to genetically identical calves, all of which carried the neomycin - marker gene.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

Since the February breakthrough, PPL Therapeutics of Edinburgh, which collaborates with the Roslin Institute, has produced five lambs from fetal cells that were genetically modified to carry marker genes and genes for human proteins.

They injected this handcrafted virus into rats and found that the marker gene was indeed active only in smooth muscle cells.

But in the 1 September issue of the Journal of Clinical Investigation, cardiologist Michael Parmacek and his colleagues at the University of Chicago describe how they deleted two genes from the common cold virus to make it unable to cause any sniffling or fever, then replaced them with a marker gene that turns out an easily detected protein and the SM22 promoter, which turns on expression of genes in smooth muscle cells that surround arteries.

«If we want to engineer the right microbiome to support plant growth, we need to understand the real function of the microbiome and not just sequence marker genes,» said study co-first author Asaf Levy, a research scientist at the JGI.

The SC3 tool contains added features that help interpret the biological function of the cells in that group, such as lists of marker genes for each group.

To see if they could selectively target the hair follicles in this cultured skin, Li and Hoffman took liposomes carrying a marker gene and applied them to the skin - bearing sponge.

After the sorting, we performed whole genome amplification on individual aggregates then sequenced the 16S rRNA marker genes to identify cells comprising the aggregate.

They also say they are concerned about the antibiotic resistance marker gene that the wheat contains, and assert that the researchers «are openly releasing a synthetic version of a compound that... has had no long - term health safety tests whatsoever for human consumption, or for its impacts on non-target species.»

The researchers could detect when RNAi occurred, because the cells would alter their normal course of cell division or fail to express the marker gene.

They also tested double - stranded RNA that matched a marker gene that they put into the fruit fly genome.

«The presence of the marker gene in the child's liver will show whether the transplantation is successful or not.

«A marker gene will be inserted into these liver cells,» says Ledley.

Eight years ago, Bishop tried to track the fate of wild - type AcNPV tagged with a «marker gene».

Hart and Vile hooked up the switch gene to the marker gene and injected the DNA directly into two types of mouse tumour.

Overexpression of wild - type ACVR1 in chick micromass cultures resulted in expression levels of marker genes that are comparable to those of the empty viral vector, while cells overexpressing R206H ACVR1 showed upregulation of collagen type II and aggrecan.

The researchers began with a simple marker gene that confers resistance to the antibiotic neomycin in bacteria.

In other tumours containing no melanocytes, the marker gene remained inactive.

Remarkably, the relative expression levels of all marker genes were only slightly decreased when R206H ACVR1 and Q207D caACVR1 cultures were cotransfected with Noggin, whereas cells expressing the wild - type ACVR1 or empty viral vector showed clear downregulation of all analyzed marker genes for chondrogenesis.

While expression levels of all marker genes remained almost unchanged in wild - type ACVR1 cultures compared with the empty viral vector, the expression profile induced by caACVR1 Q207D displayed evidence of advanced chondrogenic differentiation as shown by decreased levels of the early differentiation stage marker collagen type II together with enhanced expression of the later - stage markers Ihh and collagen type X. Indian hedgehog has been shown previously to be induced by BMP signaling transmitted via the ACVR1 receptor (9).

$ 2,500 «Beyond bacteria» Deeper shotgun metagenome and virome characterization of one sample, plus additional marker gene sequencing (16S rRNA, 18S rRNA and ITS, for you sequencing wonks) to characterize not just the bacteria but also the viruses, microbial eukaryotes (like giardia), and fungi in your gut.

Shallow shotgun metagenomics means instead of using the 16S rRNA as a marker gene, we fragment all the DNA in the sample, grind it up, sequence it in small pieces, and try to figure out what it is afterwards.

Cultured mouse, monkey, and human pluripotent cells can be driven down a retinal lineage as determined by the expression of marker genes, including the EFTFs [2]--[4].

The meeting program will encompass investigations of eukaryote biodiversity, ecology, and evolution, using approaches such as rRNA marker genes, shotgun metagenomics, metatranscriptomics, and computational biology tools and software pipelines.

AmphoraNet, AmphoraVizu - metagenomic and genomic analysis online tools using phylogenetic marker genes

Pictured Above: Greater Prairie Chicken germ - cells, fluorescing green thanks to the integration of a GFP marker gene.

Pdgfrb and Pecam1 served as pericyte and EC marker genes, respectively, whose expression was not altered (Fig. 4b).

12/8/2008 Interactive Gene «Networks» May Predict if Leukemia is Aggressive or Slow - Growing Rather than testing for individual marker genes or proteins, researchers at the University of California, San Diego (UC San Diego) and the Moores UCSD Cancer Center have evidence that groups, or networks, of interactive genes may be more reliable in... More...

Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics.

Our gut already contains billions of bacteria carrying resistances to kanamycin and ampicillin, the two most commonly used antibiotic marker genes in GM crops.

Growing genetically modified corn containing the ampicillin marker gene has no influence on abundance of this gene in soil bacteria (Demanèche and others 2008).

Therefore, it is necessary to determine the expression patterns of VASA and other germ cell marker genes in monkeys, but only limited information is currently available [7], [13].

With this reporter we split ES cell cultures into two fractions that both express certain stem cell markers but only one of which expresses low levels of an endodermal marker gene.

The expression of germ cell marker genes in monkey ES cells and tissues was examined using an RT - PCR analysis.

The expression of other germ cell marker genes, such as NANOS1, NANOS2, NANOS3 and PIWIL1, increased in the EBs as well.

This observation suggests that PIWIL1 could be a marker gene for the detection of ES cell - derived germ cells.

Panel illustrates marker genes implicated in pluripotency of NSCs, with color bar reporting log2 normalized expression values (green / red indicates high / low relative expression).

To amplify the germ cell marker genes, primers were designed based on the human sequences as shown in Table 1.

Panel below illustrates marker genes implicated in pluripotency of NSCs, with color bar reporting log2 normalized expression values (green / red indicates high / low relative expression).

However, these germ cell marker genes are not appropriate for detecting germ cell differentiation from mouse and human ES cells because these genes are expressed in both ES cells and germ cells.

To explore the differentiation markers for detecting germ cells differentiated from ES cells, the expression of various germ cell marker genes was examined in tissues and ES cells of the cynomolgus monkey (Macaca fascicularis).

CXCR4, which is a chemokine receptor, is expressed in migratory and post-migratory germ cells in vivo in mice [27], and is also a marker for germ cells derived from human ES cells [28], thus suggesting that CXCR4 is a candidate marker gene for detecting ES cells - derived germ cell in monkeys.

(A) The expression patterns of germ cell marker genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined using an RT - PCR analysis.

Our analysis of 23,184 cells identifies 15 clusters, few of which could be fully characterised by a single marker gene.

(A) Expression of 5 stem marker genes in single cells isolated from the edge (E), mid (M) or adjacent center (C) region of HES2 colonies.

Data are normalized to cyclophilin and expressed as ΔCt (marker gene — cyclophilin).

Marker genes expressed at very low or undetectable levels (Ct 35 — 40) fall above the upper dotted line.

Say a number of genes are correlated in both cell types, but in one cell type, some marker genes are shifted.