Sentences with phrase «radioimmunoprecipitation assay»

Recently, a radioimmunoprecipitation assay was developed to detect antibodies to recombinant canine glutamic acid decarboxylase and insulinoma antigen - 2 (84,85).

Cultured cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 μl RIPA per 1 × 106 cells; 50 mM Tris / HCl; 150 mM NaCl, 1 % Triton X-100, 1 mM EDTA, 1 mM NaF, 1 mM Na3VO4, 1 μg / ml leupeptin and 1 μg / ml pepstatin) for 1 h on ice, and insoluble debris was removed by centrifugation.

Cells were lysed 48 h later with modified radioimmunoprecipitation assay buffer (RIPA buffer, 50 mM Tris - HCl, pH7.4, 150 mM NaCl, 1 % Nonidet P - 40 (NP - 40), 0.25 % sodium deoxycholic acid, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM phenylmethylsulphonyl fluoride (PMSF), 1X Complete cocktail protease inhibitor (Roche, Penzberg, Germany)-RRB-.

Abbreviations: ACVR2A, activin A receptor type IIA; BMP, bone morphogenetic protein; BMPR, BMP receptor, type II; CNS, Central nervous system; DA, dopaminergic; DMEM / F12, Dulbecco's modified Eagle's medium nutrient mixture F - 12; E, embryonic day; GDF, growth differentiation factor; GO, gene ontology; KEGG, Kyoto encylopedia of genes and genomes; MAPK, Mitogen - activated protein kinase; mDA, midbrain dopaminergic; PD, Parkinson's disease; RIPA, radioimmunoprecipitation assay; SN, Substantia nigra; TGF - β, transforming growth factor - β; TH, tyrosine hydroxylase; VM, ventral midbrain / mesencephalon; Zeb2, Zinc finger E-box-binding homoeobox 2

Cells were lysed in radioimmunoprecipitation assay buffer with protease and phosphatase inhibitors.

The presence of antibodies to lymphadenopathy - associated retrovirus (LAV) was determined by a radioimmunoprecipitation assay and by an enzyme - linked immunosorbent solid assay of sera from Zairian patients with the acquired immune deficiency syndrome (AIDS) in 1983.