Sentences with phrase «reaction mixture»

PCR was performed in a 50 μl reaction mixture using Titanium TaqDNA polymerase (BD Biosciences, Palo Alto, CA).

Moreover, only small amounts of a single green organic solvent are needed for extracting the final product, which means that the remaining reaction mixture can be used repeatedly.

Chemists regularly wish they could perform such tricks, says Julian Eastoe, a chemist at the University of Bristol in the UK — such as after adding expensive catalytic nanoparticles to an industrial reaction mixture.

The Quantitect One - Step RT - PCR kit (Qiagen, Hilden, Germany) was used with a 25 μl reaction mixture under the following conditions: 0.25 μl of kit enzyme mixture (including reverse transcriptase RT and Taq polymerase), 10 μl of 2 × Quantitect RT - PCR buffer, 1.25 μl of 10 μM of each primer, 0.5 μl of 10 μM of probe at 10 μM, 6.8 μl of DNA RNA free water (Mol Bio grade, Hamburg, Germany) and 5 μl of the extracted sample.

This unstable hydrazone (50 g, 172.41 mmol) was dissolved in CH2Cl2 (50 ml), Et3N (57.56 mL, 413.79 mmol) followed by (CF3CO) 2O (28.77 mL, 206.89 mmol), was added at 0 °C and the mixture was stirred at room temperature for 1 h. Reaction mixture was diluted with aq.

With MagSi - Direct, you can start with a molecule of interest, attach magnetic beads to it and then use a magnet to isolate that molecule from any desired reaction mixture, together with any other molecules, complexes or even intact cells to which the starting molecule has bound.

Diaminoglyoxime is shown in the foreground, following filtration and drying from the reaction mixture.

But until recently, it was not possible to probe gas temperatures in the reaction mixture without at the same time perturbing the flow inside an operating reactor.

«It can inactivate 15 - PGDH when added at one part in 10 billion into a reaction mixture, which means it has promise to work as a drug.»

By adding an (NHTs) − salt to the reaction mixture, the authors could shift the ratio almost completely in favor of the C - N elimination pathway.

After printing the DNA on the array the latter is submerged in a reaction mixture that synthesizes the proteins specified by the printed DNA.

The reactions were stopped after 15 min by aspirating the reaction mixture and rapidly rinsing each well four times with PBS at 4 °C.

The reaction mixture was diluted with 150 ml of water and extracted with ethyl acetate (3 × 100 ml), the combined organic layer was washed with saturated brine (100 ml), dried over MgSO4, filtered, and concentrated to residue oil.

The reaction mixture was diluted with 150 ml of water and extracted with DCM (3 × 100 ml).

The reaction mixture was diluted with 100 ml of water and extracted with ethyl acetate (3 × 100 ml), combined organic layer was washed with saturated brine (100 ml), dried over MgSO4, filtered, and concentrated.

The valuable alcohols are easily separated from the reaction mixture in a process that promotes the re-use of the major components of the process, furthering the efficiency and reducing the potential waste generation of the process.