Sentences with phrase «read sequencing methods»

Not exact matches

This new sequencing approach can be used for any DNA that is too fragmented to be read well through more traditional methods.

The new study, led by Johannes Krause, a geneticist at the Max Planck Institute for the Science of Human History in Jena, Germany, used next - generation sequencing methods to read stretches of any DNA present in a sample and fish out those that resembled human DNA.

The single - cell technique is better at detecting subtle mosaicism than usual DNA sequencing methods, which sequence many thousands or millions of cells mixed together and read out an average for the sample.

Using this method, the researchers sequenced enough reads to cover the entire length of the Y chromosome about 450 times.

Then in the early 1990s, Katherine Cullen and a team at Vanderbilt University developed a method to artificially fuse pieces of DNA that are nearby in the nucleus — a seminal feat that made it possible to analyze the ultrafolded structure of DNA merely by reading the DNA sequence.

And they figured they could read the address using methods akin to next - generation DNA sequencing, a set of high - speed genome sequencing methods Church helped develop in the early 2000s.

The third is a method implemented by Reich for reading the genetic codes of 1.2 million carefully chosen variable parts of DNA (known as single nucleotide polymorphisms) rather than having to sequence entire genomes.

Modern genome sequencing methods make it relatively easy to read the individual «letters» in DNA, but only in short fragments.

DIGGING FOR DNA See full infographic: JPG © LUCY READING - IKKANDAIn addition to the DNA extraction and isolation improvements, Meyer and his colleagues accomplished the unprecedented feat by using a homegrown single - stranded library preparation method to ready the DNA for sequencing.

Using simulations, we show the method is accurate across a variety of read lengths, with different length reference sequences, at different sample depths, and when samples contain reads originating from organisms absent from the reference.

Our method, based ideally on 20x and 50x of NaS and Illumina reads respectively, provides an efficient and cost - effective way of sequencing microbial or small eukaryotic genomes in a very short time even in small facilities.

The method enables use of widely available short - read DNA sequencing platforms to study long single molecules within a complex sample, without losing information of physical connection between sequencing reads from each molecule of origin.

We mapped sequencing reads to the Nvit1.0 assembly [15] using a TopHat2 [45] and Stampy [46], and then mapped reads which failed to align to the genome against the OGSv2 predicted transcriptome with bowtie2 [47] to improve mapping sensitivity (see methods for details).

During the pilot phase, the Project is analysing Illumina short - read sequence data on 2,512 samples from multiple locations in Africa and Asia, together with laboratory samples for benchmarking and methods development.

Here, we present a method, called MrMosaic, to detect structural mosaic abnormalities using deviations in allele fraction and read coverage from next - generation sequencing data.

Genome sequencing has become a powerful tool for studying emerging infectious diseases; however, genome sequencing directly from clinical samples without isolation remains challenging for viruses such as Zika, where metagenomic sequencing methods may generate insufficient numbers of viral reads.

Unlike other methods, which read chunks of DNA perhaps a hundred or so base pairs in length, the MinION can read sequences as long as 882,000 base pairs in length using the nanopore technology.

Read and interpret blueprints, sketches, and / or product specifications to determine sequence and methods of fabrication, assembling, and installing products.

• Able to read and interpret sketches, blueprints and isometric plans and drawings to lay out piping plans • Competent in planning the sequence of installations in order to avoid obstructions • Hands - on experience in installing pipe and related materials and equipment, including supports, hangers and hydraulic cylinders • Determines required equipment and welding methods by applying expertise in metallurgy and geometry