Sentences with phrase «read sequencing technologies»

Genome assemblies obtained from short read sequencing technologies are often fragmented into many contigs because of the abundance of repetitive sequences.

Long - read sequencing technologies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing reads with average fragment lengths of over 10,000 base - pairs and maximum lengths reaching 100,000 base - pairs.

Long - read sequencing technologies were launched a few years ago, and in contrast with short - read sequencing technologies, they offered a promise of solving assembly problems for large and complex genomes.

Long - read sequencing technologies are transforming our ability to assemble highly complex genomes.

To overcome the extreme genomic complexity, the team used new long - read sequencing technology that boosted the quality of the genome sequence obtained by more than one hundred fold over standard short - read approaches.

Eichler predicted, «In five years there might be a long - read sequence technology that will allow clinical laboratories to sequence a patient's chromosomes from tip to tip and say, «Yes, you have about three to four million SNPs and insertions deletions but you also have approximately 30,000 - 40,000 structural variants.

Not exact matches

The UW team has been exploring nanopore technology to read DNA sequences quickly.

A technology that simultaneously reads a DNA sequence and its crucial modifications makes its debut.

One sequencing technology used by the researchers produces massive amounts of very short reads — about 150 to 250 bases in length.

The Y chromosome, like all DNA, is composed of a series of molecules called «bases» that are represented by the letters A, T, C, and G. Current genetic sequencing technologies produce «reads» of sequence that are much shorter than the entire length of the chromosome.

«By reducing non-Y chromosome reads from our data with flow sorting and the RecoverY technique that we developed, and by using this combination of sequencing technologies, we were able to assemble the gorilla Y chromosome so that more than half of the sequence data was in chunks longer than about 100,000 bases in length,» said Medvedev.

A study of this scope is possible thanks to innovative techniques for in vitro selection (such as mRNA display), as well as a revolutionary technology permitting massively parallel RNA sequencing (known as deep sequencing), which provides unprecedented speed and read accuracy.

To retrieve their files, they used modern sequencing technology to read the DNA strands, followed by software to translate the genetic code back into binary.

Massively parallel sequencing technologies, while increasing the speed, improving the accuracy, and reducing the cost of genome sequencing, typically produce only short stretches of sequences called «reads» After sequencing, the reads are pieced back together with genome assembly software.

A new sequencing technology based on longer sequence reads allows missing genes and missing forms of genetic variation to be discovered for the first time.

The researchers used Single Molecule, Real - Time (SMRT) sequencing technology, the assembly tools Falcon and QUIVER, and other techniques to generate long sequence reads.

Current technology for «long read» detailed genomic sequencing can be performed using expensive instrumentation (around # 500,000).

The SMRT technology used in the new study makes it possible to sequence and read DNA segments longer than 5,000 bases, far longer than standard gene sequencing technology.

Recent and rapid advances in technologies that permit large - scale creation and synthesis («writing») of longer pieces of synthetic DNA, as well as the advent of extremely fast, cheap and accurate sequencing («reading») of DNA, have changed our collective thinking about the feasible size and scope of projects in many labs.

In addition to different variant annotation approaches (of which there are more than we have compared here), there are different sequencing technologies, read mappers and variant callers.

We refer interested readers to systematic comparisons of other aspects of the next - generation sequencing pipeline, for example comparisons of benchtop high - throughput sequencing technologies [56], short - read mappers [57], variant callers [58] and variant - calling pipelines as a whole [59, 60].

Here, we describe the results obtained from our current long read sequencing project of 100 Icelanders with Oxford Nanopore technology.

And to do this, she used the latest technology to sequence the DNA — map its building blocks to read its genes.

Stephen R. Quake, Ph.D., Stanford University, Palo Alto, Calif. $ 1.8 million (3 years) «High - Throughput, Single - Molecule DNA Sequencing» This group will try to improve its sequencing - by - synthesis technology in order to achieve longer reads from very large numbers of single DNA Sequencing» This group will try to improve its sequencing - by - synthesis technology in order to achieve longer reads from very large numbers of single DNA sequencing - by - synthesis technology in order to achieve longer reads from very large numbers of single DNA molecules.

New generation of sequencing technology uses nanopores to deliver ultra long read length single molecule sequence data, at competitive accuracy, on scalable electronic GridION platform.

Fortunately, indel detection is one area that will be helped dramatically by improvements to the sequencing technologies, namely longer reads and paired - end protocols.

Motivation: Single Molecule Real - Time (SMRT) sequencing technology and Oxford Nanopore technologies (ONT) produce reads over 10kbp in length, which have enabled high - quality genome assembly at an affordable cost.

The long sequencing reads produced by Oxford Nanopore's platforms enable the assembly of genomes with superior contiguity compared to those produced by second generation technologies.

Although such technologies are capable of generating vast amounts of sequencing data, the short read length makes it more difficult to assign how individual reads relate to each other.

Despite improvements in genomics technology, the detection of structural variants (SVs) from short - read sequencing still poses challenges, particularly for complex variation.

Modern DNA sequencing technologies predominantly produce short read sequences.

Speed, single - base sensitivity and long read lengths make nanopores a promising technology for high - throughput sequencing.

The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it.

Short - read, high - throughput sequencing technology can not identify the chromosomal position of repetitive insertion sequences that typically flank horizontally acquired genes such as bacterial virulence genes and antibiotic resistance genes.

Unlike other methods, which read chunks of DNA perhaps a hundred or so base pairs in length, the MinION can read sequences as long as 882,000 base pairs in length using the nanopore technology.

Current massively parallel DNA sequencing technologies produce short sequence reads that are often unable to resolve haplotype information.

Oryza coarctata plants, collected from Sundarban delta of West Bengal, India, have been used in the present study to generate draft genome sequences, employing the hybrid genome assembly with Illumina reads and third generation Oxford Nanopore sequencing technology.

We further determined sequencing accuracy and compared the assembly to short - read technologies.

Complementary sequencing using long - read technology will later be performed for regions of high medical importance.

The organizers of the project, called GP - write (for work in model organisms and plants) or sometimes HGP - write (for work in human cell lines), envision it as a successor to the Human Genome Project (retroactively termed HGP - read), which 25 years ago promoted rapid advances in DNA sequencing technology.

Built for each grade level and customized for each high - stakes state assessment, the ELA Test Challenge courses help students develop strength and stamina for reading complex informational texts and practice with technology - enhanced items, including drag - and - drop sequencing, click - to - highlight evidence tasks, multi-part items, and multi-select multiple - choice questions.

The company has been heavily invested in PRISM technology which allows the display to switch colors in nearly any pattern, shape, speed and sequence, making it the ideal technology to transform the commercial,... [Read more...]

DNA lasts for around 2000 years if stored at around 10 degrees centigrade, and can be read using existing gene sequencing technology.