Sentences with phrase «reagents from»

Clinical chemistry assessment was performed using a Beckman - Coulter AU 480 autoanalyzer and reagents from Beckman - Coulter (except free fatty acids, which were measured using kit NEFA - HR2 from Wako Chemicals, and glycerol, which was measured using a kit from Randox Laboratories) as previously described (46, 47).

Is it ever acceptable to turn down a request for published data or reagents from another academic scientist?

Not exact matches

Still, in some ways I do feel scientifically isolated from Europe working here, and I am reminded of the geographical distance when ordering reagents takes weeks to come from the United States or Europe.

I mean, obviously no one has created a living cell from buying reagent grade chemicals, so where does the research stand right now?

We go to the lab, solve problems, write papers, order reagents, steal bagels from seminars, and go home.

Support from such a fund might be just what you need to pay for minimal travel (to support collaborations) and basic supplies and reagents.

Several companies now sell reagents and equipment for isolating RNA from individual cells while keeping the thousands of resulting samples separate.

Dr. Uhlén founded the Science for Life Laboratory in Stockholm, Sweden, where he served as director from 2010 to 2015, and has authored more than 750 publications in bioscience with a focus on the development and use of affinity reagents in biotechnology and biomedicine.

Dluzen explains that his lab is fairly big and as a result, there is a lab manager who orders reagents and gets final approval from the PI.

Meeting attendance policy, attendance expectations, designation of common areas, safety regulations, rotating lab jobs, reagent - reordering procedures, and keeping track of stocks — these are all examples of organizational situations that benefit from rules — from structure.

Scientists commissioned by the programme contacted Skuse for the exact recipe of his modified Griess reagent, and when they used it they obtained quite different results from his.

The process involving liquid chromatography - electrospray ionization - tandem mass spectrometry relies on a vital step called «charge derivatization» or using a permanently charged reagent to selectively trap the estrogens and isolate them from the lipids and proteins that could interfere with estrogen detection, Schug said.

Ernesto Rodriguez Leal, native from Monterrey, in northern Mexico, and a PhD in mechanical engineering, told us that the ring is placed only at the time of the test, a button is pressed and activates a needle that draws blood, which is transported by capillary action and taken to a lab - on - a-chip, a recent concept based on immobilizing reagents to look for changes and make measurements.

If we can't afford reagents I beg / borrow / steal from other labs at our big med school.

Unlike conventional fingerprint visualization reagents, the polymers used by the University of Leicester researchers are electrochromic, that is to say they change from one colour to another when subjected to an electrical voltage.

«We're isolating our scientists from the rest of the world» by preventing them from sharing reagents, Bertuzzi said.

HGS, he claims, is ready to share data and reagents with them: «We would not block anyone in the academic world from using this for research purposes.»

Secondary reagents included goat antirat horseradish peroxidase and goat antirabbit peroxidase - conjugated F (ab ′) 2 fragments both from Jackson ImmunoResearch (West Grove, PA) and Alexa fluorescent 594 antigoat IgG and Alexa fluorescent 594 goat antirabbit complex from Molecular Probes (Eugene, OR).

Total RNA was extracted from frozen adipose tissue (100 mg), FACS - isolated cells (> 105), or cultured cells (60 - mm confluent plate) using a commercially available acid - phenol reagent (TRIzol; Invitrogen Corp.).

These efforts to produce simple organic compounds from simple reagents heralded the dawn of prebiotic organic chemistry.

Endothelial cells derived from the brain demonstrated a complete absence of uptake after 4 h of incubation (at 33 °C or 37 °C) with this fluorescent reagent (data not shown).

Total RNA was extracted from the perigonadal (epididymal or parametrial) adipose tissue of individual mice using a commercially available acid - phenol reagent (TRIzol; Invitrogen Corp., Carlsbad, California, USA).

Isolate RNA from each collected cell population using TRI reagent following manufacturer's instructions.

Time and money savings come from the fact that CRISPR is so efficient that you can inject the reagents directly into fertilized mouse eggs, circumventing the need for enrichment and selection offered by mouse ESCs.

She loves that her job allows her to interact with scientists from all over the world and help them easily share their reagents.

Total RNA was extracted from the whole brains of Japanese honeybee workers with seizures induced by awakening from anesthesia, using TRIzol Reagent (Invitrogen, Carlsbad, CA).

Academic and non-academic research groups, including SMEs, can apply to benefit from the expertise, reagents, and facilities offered by TRANSVAC2 to accelerate the development of their vaccines.

For the 2018 awards, we've widened the remit of the coveted Researchers Choice award to include suppliers from across numerous reagent types.

Human total RNA was also extracted using TRIzol ® Reagent (Invitrogen) from peripheral blood lymphocytes and fat tissue.

AMSBIO offers a range of kits and reagents for the extraction (isolation) of high quality RNA from FFPE tissues and LCM samples that can be used not only for PCR but also microarray applications.

You may want to declare your bench «RNase free» and refrain from talking to friends and colleagues while working with these reagents.

Purified T cells from the spleen were labeled with CellTrace CFSE (Invitrogen, Carlsbad, CA) using protocols and reagents supplied by the manufacturer.

Reagents: antibodies, plasmids, cell lines, RNA libraries, and more Organisms and viruses: model organisms of disease, mouse models, and more Biological specimens Software Protocols Core laboratories eagle - i is different from traditional web publications that list resources (websites, journals, etc.) because the tool allows researchers to: Share resources

Total RNA was isolated from the S. pistillata fragments by using TRIzol ® reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer's instructions.

Efficient generation of megakaryocytes from human induced pluripotent stem cells using food and drug administration - approved pharmacological reagents.

Learn about how our platform is optimized for gRNAs from several leading providers, using a range of transfection reagents.

The Persomics platform has been optimized for gRNAs / siRNAs from several leading providers and a range of transfection reagents.

This reagent was prepared from discarded HIV - positive units of blood collected from the National Blood Bank in Kampala, Uganda.

Review of «Efficient Generation of Megakaryocytes From Human Induced Pluripotent Stem Cells Using Food and Drug Administration - Approved Pharmacological Reagents» from Stem Cells TM by Stuart P. AtkiFrom Human Induced Pluripotent Stem Cells Using Food and Drug Administration - Approved Pharmacological Reagents» from Stem Cells TM by Stuart P. Atkifrom Stem Cells TM by Stuart P. Atkinson

As in the first edition of this book, the laboratory course is completely supported by quality - assured products from the Carolina Biological Supply Company, from bulk reagents, to useable reagent systems, to single - use kits, thus satisfying a broad range of teaching applications.

Reagents for performing these assays, under the name TotalSeq ™ are now available from BioLegend.

Normal serums are recommended as a blocking reagent to reduce background from non-specific, conserved sequence and / or Fc - receptor binding.

All starting materials, chemicals and reagents were obtained from Sigma Aldrich, (Milwaukee, WI, USA), and were used as received.

For microRNA (miRNA) analysis, total RNAs, including miRNAs, were isolated from the cell lines using TRIzol reagent (Invitrogen Corporation) in accordance with the manufacturer's instructions.

Solvents used for synthesis and chromatographic analysis were HPLC or ACS reagent grade and were purchased from Fisher Scientific Co (Pittsburg, PA, USA).

These solutions range from CDx biomarker identification and patient stratification to assay development and reagent manufacturing, to the commercialization of these assays either as services at our harmonized clinical laboratories or as kits available to laboratories in over 65 countries.

An online lab notebook, Silencing Genomes combines lab methods with user - friendly features adapted from the DNALC's popular text DNA Science - including flow charts, reagent recipes, and extensive instructor information.

Magnetic «bait» reagents may be constructed from proteins, cytokines, carbohydrates, pharmaceuticals, aptamers or nearly any other class of molecule.

Efficient Production of the Liquid Fuel 2,5 - Dimethylfuran from Fructose Using Formic Acid as a Reagent, Todsapon Thananatthanachon and Thomas Rauchfuss, Angewandte Chemie International Edition, DOI: 10.1002 / anie.201002267, August 2, 2010.

All reagents were from Invitrogen (Invitrogen, Carlsbad, CA) unless stated otherwise.